Amplification primers of cox3 genes of razor clam type mitochondria frequently seen in northern part of China and screening method of amplification primers

Abstract

The invention relates to amplification primers of cox3 genes of razor clam type mitochondria frequently seen in the northern part of China and a screening method of the amplification primers. The sequence of the amplification primer cox3CF is 5'TATCATTTAGTHGATATAAGACC 3', and the sequence of the amplification primer cox3CR is 5'CCATGAAADCCAGTTATYACAA 3'. A preparation method of the amplification primers includes the five steps of sequence downloading, sequence comparing, primer designing and synthesizing, DNA extracting and primer amplifying. The pair of primers cox3CF and cox3CR for amplifying part of the cox3 sequence of razor clam type mitochondria frequently seen in the northern part of China are screened out, the reaction system and PCR amplification conditions of the primers are determined, and it is proved through experiments that the primers have the advantages of being simplex in stripe and high in amplification efficiency and can meet the requirements for razor clam type mitochondrial genome research and subsequent genetic diversity and systemic embryology research.

Description

technical field [0001] The present invention relates to a shellfish mitochondrial cox3[cytochrome c Oxidase subunit III (cytochrome c oxidasesubunitIII)] gene amplification primers, in particular relate to a kind of amplification primers and screening methods for common northern razor clam mitochondrial cox3 genes. Background technique [0002] Razor clams belong to the phylum Mollusca, Bivalvia, Heterodonta, and Veneroida. They live in the intertidal zone to hundreds of meters deep and are common economic shellfish. kind. Especially large razor clam, long razor clam, small knife razor clam and hanging razor clam are widely distributed in my country and are one of the favorite marine shellfish of the coastal masses of my country, occupying an important position in edible shellfish. [0003] In recent years, due to overharvesting, deteriorating marine environment and shrinking habitat, the resources of razor clams have been declining year by year. It is urgent to speed up ...

AI Technical Summary

Problems solved by technology

In the process of measuring the mtDNA of razor clam by walking method, a pair of Long-PCR primers designed using short fragments of cox1 gene and 16SrRNA gene failed to amplify successfully, which hindered the acquisition of full-length