PCR method of raillietina echinobothrida identification

A technique for sharpening tapeworms and spines, which is applied in the field of identification of tapeworm species of the genus Laili, can solve the problems of inaccurate judgment, long time-consuming, complicated operation, etc., and achieve the effect of small workload, single band, and simple result judgment

Active Publication Date: 2020-08-11
SHANXI AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Reilia echinosum is the largest species of Reilly genus that threatens chicken health and breeding. At present, the identification of Reilly echinosum still relies on the traditional morphological characteristics of the worm, which is time-consuming, complicated, and inaccurate. shortcoming

Method used

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  • PCR method of raillietina echinobothrida identification
  • PCR method of raillietina echinobothrida identification
  • PCR method of raillietina echinobothrida identification

Examples

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Embodiment 1

[0045] A PCR method for the identification of Reilia echinosum, which provides two gene detection primers of 18s rDNA and ITS-2 rDNA for the identification of Reilly echinosum species, said method comprising the following steps:

[0046] Step 1. Collection and morphological identification of Taenia echinosum: Collect cases of chickens infected with tapeworms, kill the sick chickens by bleeding, take out the complete digestive tract, carefully cut open the intestines, and put the found tapeworms together with the intestines in clean water , after the tapeworm cephalome detached from the intestinal mucosa and fully stretched, it was washed in normal saline, and each worm body was stored separately after cleaning. The body was fixed in 2.5% glutaraldehyde aqueous solution (the formula is: 10mL volume fraction is 25% glutaraldehyde aqueous solution, add 50mL 0.2M phosphate buffer and 40mL distilled water, adjust pH=7.4, add distilled water to make up to 100mL, wherein The formula ...

Embodiment 2

[0061] A PCR method for the identification of Reilia echinosum, which provides two gene detection primers of 18s rDNA and ITS-2 rDNA for the identification of Reilly echinosum species, said method comprising the following steps:

[0062]Step 1. Collection and morphological identification of Taenia echinosum: Collect cases of chickens infected with tapeworms, kill the sick chickens by bleeding, take out the complete digestive tract, carefully cut open the intestines, and put the found tapeworms together with the intestines in clean water , after the tapeworm cephalome detached from the intestinal mucosa and fully stretched, it was washed in normal saline, and each worm body was stored separately after cleaning. The body was fixed in 2.5% glutaraldehyde aqueous solution (the formula is: 10mL volume fraction is 25% glutaraldehyde aqueous solution, add 50mL 0.2M phosphate buffer and 40mL distilled water, adjust pH=7.4, add distilled water to make up to 100mL, wherein The formula o...

Embodiment 3

[0077] A PCR method for the identification of Reilia echinosum, which provides two gene detection primers of 18s rDNA and ITS-2 rDNA for the identification of Reilly echinosum species, said method comprising the following steps:

[0078] Step 1. Collection and morphological identification of Taenia echinosum: Collect cases of chickens infected with tapeworms, kill the sick chickens by bleeding, take out the complete digestive tract, carefully cut open the intestines, and put the found tapeworms together with the intestines in clean water , after the tapeworm cephalome detached from the intestinal mucosa and fully stretched, it was washed in normal saline, and each worm body was stored separately after cleaning. The body was fixed in 2.5% glutaraldehyde aqueous solution (the formula is: 10mL volume fraction is 25% glutaraldehyde aqueous solution, add 50mL 0.2M phosphate buffer and 40mL distilled water, adjust pH=7.4, add distilled water to make up to 100mL, wherein The formula ...

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Abstract

The invention specifically relates to a PCR method of raillietina echinobothrida identification, and belongs to the field of the identification of the species of raillietina echinobothrida infecting poultry. The raillietina echinobothrida is the largest species of raillietina tapeworms that threaten the health and breeding of chickens; and at present, the identification of the raillietina echinobothrida still depends on the difference analysis of traditional worm morphological characteristics, so that disadvantages of long duration time, complex operation and inaccurate judgment exist. Throughthe reference of the published ribosomal gene sequences of the raillietina tapeworms, 18s rDNA and ITS-2 rDNA primers can be autonomously designed; through the optimization of the PCR method, the 18srDNA and ITS-2 rDNA gene products of the raillietina echinobothrida can be accurately amplified; and therefore, the 18s rDNA and ITS-2 rDNA double-gene confirmation based PCR method capable of rapidly and accurately identifying the raillietina echinobothrida can be established.

Description

technical field [0001] The invention specifically relates to a PCR method for identifying Taenia echinosum, and belongs to the field of identification of Taenia reili species that infects poultry. Background technique [0002] Taeniasis is a parasitic disease that seriously endangers chicken health and breeding industry. The tapeworms that parasitize chickens belong to the genus Reilly of the platyhelminth phylum, cestode class, round leaf order, and Devonidae. Tapeworms of the genus Reilly parasitize in the small intestines of various poultry such as chickens, turkeys, and pheasants. Through nutrient absorption and release of toxins, chickens cause developmental retardation, slow growth, emaciation, anemia, poisoning and other symptoms. A large number of tapeworm parasitism can also cause chicken intestinal obstruction, induce intestinal obstruction and acute inflammation. Chickens can be infected with one tapeworm alone or with a mixture of several tapeworms. In recent ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6869C12Q1/6888
CPCC12Q1/6869C12Q1/6888C12Q2531/113C12Q2565/125Y02A50/30
Inventor 张鼎刘晶英杨述远田文霞
Owner SHANXI AGRI UNIV
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