Molecular marker assisted breeding primer based on growth traits of strongylocentrotus intermedius and screening method

A molecular marker-assisted and growth trait technology, applied in the field of molecular biology, can solve the problem of fewer detection sites and achieve high accuracy

Active Publication Date: 2018-10-16
DALIAN OCEAN UNIV
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Problems solved by technology

[0003] Chang Yaqing used HRM technology to screen specific SNP genotypes of sea urchins for molecular assisted breeding in the early screening of SNP primers and screening methods for improved sea urchins. However,

Method used

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  • Molecular marker assisted breeding primer based on growth traits of strongylocentrotus intermedius and screening method
  • Molecular marker assisted breeding primer based on growth traits of strongylocentrotus intermedius and screening method
  • Molecular marker assisted breeding primer based on growth traits of strongylocentrotus intermedius and screening method

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Embodiment

[0034] 1. SLAF tag screening for growth traits

[0035]The present invention utilizes the SLAF-seq technology to carry out gene sequencing on the sea urchin mesenterus, and obtains a large number of SLAF tags in the sequencing, and the SLAF tags can be used to construct a high-density genetic map, and utilizes the genetic map and the phenotypic traits of the sea urchin mesenterus (shell height, shell diameter) , body weight) for association analysis and QTL mapping, through the QTL screening of the target traits (shell height, shell diameter, body weight), the SLAF tags related to growth traits (shell height, shell diameter, body weight) can be deduced back. Among them, SLAF-seq technology and HighMap software were used to develop and construct genetic maps, and the interval mapping (IM) algorithm of MapQTL software and the composite interval mapping (CIM) algorithm of RQTL software were used for QTL positioning. Specifically, for the method of constructing the genetic map of ...

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Abstract

The invention discloses a molecular marker assisted breeding primer based on growth traits of strongylocentrotus intermedius and a screening method and relates to the field of molecular biology. An SLAF-seq technology and a KASP technology are mainly adopted for related SNP marking of growth traits of Strongylocentrotus, and the SNP marking is applicable to early assisted seed selection of the strongylocentrotus intermedius. Through the SLAF-seq technology, accurate typing is guaranteed through high-depth sequencing, the sequencing cost is reduced through a simplified strategy, and the optimalmarking number is guaranteed through prediction of a previous simplification scheme; through the KASP technology, the operation time is shortened, steps are simplified and the cost is reduced.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a molecular marker-assisted breeding primer and a screening method for the growth traits of the sea urchin intermedia. Background technique [0002] At present, traditional breeding methods such as crossbreeding and selective breeding are mostly used for the breeding of sea urchins intermedia. No matter it is crossbreeding or selective breeding, the parents are selected based on sea urchins with good growth characteristics. These methods all need to measure its growth traits (shell height, shell diameter, body weight) regularly during the growth process of sea urchins, and use this as a standard for step-by-step screening. These selection methods not only consume a lot of manpower and material resources, but also Can cause the progeny seed output of good genotype to be low because the gene of parent is not good gene. Molecular marker-assisted breeding can improve the efficiency ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 常亚青丁君李磊张伟杰赵文飞江会杰
Owner DALIAN OCEAN UNIV
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