Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Molecular marker assisted breeding primer based on growth traits of strongylocentrotus intermedius and screening method

A molecular marker-assisted and growth trait technology, applied in the field of molecular biology, can solve the problem of fewer detection sites and achieve high accuracy

Active Publication Date: 2018-10-16
DALIAN OCEAN UNIV
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Chang Yaqing used HRM technology to screen specific SNP genotypes of sea urchins for molecular assisted breeding in the early screening of SNP primers and screening methods for improved sea urchins. However, this method only involves one SNP site related to growth traits. Point detection, for the good genes that control quantitative traits, there are fewer detection sites, which has certain limitations in application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Molecular marker assisted breeding primer based on growth traits of strongylocentrotus intermedius and screening method
  • Molecular marker assisted breeding primer based on growth traits of strongylocentrotus intermedius and screening method
  • Molecular marker assisted breeding primer based on growth traits of strongylocentrotus intermedius and screening method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0034] 1. SLAF tag screening for growth traits

[0035]The present invention utilizes the SLAF-seq technology to carry out gene sequencing on the sea urchin mesenterus, and obtains a large number of SLAF tags in the sequencing, and the SLAF tags can be used to construct a high-density genetic map, and utilizes the genetic map and the phenotypic traits of the sea urchin mesenterus (shell height, shell diameter) , body weight) for association analysis and QTL mapping, through the QTL screening of the target traits (shell height, shell diameter, body weight), the SLAF tags related to growth traits (shell height, shell diameter, body weight) can be deduced back. Among them, SLAF-seq technology and HighMap software were used to develop and construct genetic maps, and the interval mapping (IM) algorithm of MapQTL software and the composite interval mapping (CIM) algorithm of RQTL software were used for QTL positioning. Specifically, for the method of constructing the genetic map of ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a molecular marker assisted breeding primer based on growth traits of strongylocentrotus intermedius and a screening method and relates to the field of molecular biology. An SLAF-seq technology and a KASP technology are mainly adopted for related SNP marking of growth traits of Strongylocentrotus, and the SNP marking is applicable to early assisted seed selection of the strongylocentrotus intermedius. Through the SLAF-seq technology, accurate typing is guaranteed through high-depth sequencing, the sequencing cost is reduced through a simplified strategy, and the optimalmarking number is guaranteed through prediction of a previous simplification scheme; through the KASP technology, the operation time is shortened, steps are simplified and the cost is reduced.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a molecular marker-assisted breeding primer and a screening method for the growth traits of the sea urchin intermedia. Background technique [0002] At present, traditional breeding methods such as crossbreeding and selective breeding are mostly used for the breeding of sea urchins intermedia. No matter it is crossbreeding or selective breeding, the parents are selected based on sea urchins with good growth characteristics. These methods all need to measure its growth traits (shell height, shell diameter, body weight) regularly during the growth process of sea urchins, and use this as a standard for step-by-step screening. These selection methods not only consume a lot of manpower and material resources, but also Can cause the progeny seed output of good genotype to be low because the gene of parent is not good gene. Molecular marker-assisted breeding can improve the efficiency ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 常亚青丁君李磊张伟杰赵文飞江会杰
Owner DALIAN OCEAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com