Kit and application thereof in detecting vascular endothelial growth factors in anterior chamber fluid
A kit and reagent technology applied to the detection of vascular endothelial growth factor in the anterior chamber fluid. In the field of kits, it can solve the problems of long detection time, narrow linear range, and high sample demand, and meet the sample demand. Small, wide linear range, time-saving effects
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[0072] (1) Preparation of enzyme reactants
[0073] (a) Antibody treatment
[0074] PD10 column equilibration: Equilibrate the PD10 column with TSE85 buffer, 5 mL each time, repeat 5 times;
[0075] Antibody loading: Take the vascular endothelial growth factor-specific antibody for labeling (trade name 11066-R010, purchased from Sino Biological Company), the mass is 1 mg, the antibody concentration is 1 mg / mL, and the volume of the antibody needs to be 1000 μL; the antibody is added to the equilibrated For the PD10 column, add 1500 μL of TSE85 buffer;
[0076] Antibody recovery: Add 3mL of TSE85 buffer to the PD10 column and recover the antibody by gravity;
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