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Kit and application thereof in detecting vascular endothelial growth factors in anterior chamber fluid

A kit and reagent technology applied to the detection of vascular endothelial growth factor in the anterior chamber fluid. In the field of kits, it can solve the problems of long detection time, narrow linear range, and high sample demand, and meet the sample demand. Small, wide linear range, time-saving effects

Pending Publication Date: 2021-03-26
杭州华视诺维医疗科技有限公司
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AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to overcome the defects of high sample demand, long detection time and narrow linear range in the existing detection technology for diagnosing VEGF-related diseases

Method used

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  • Kit and application thereof in detecting vascular endothelial growth factors in anterior chamber fluid
  • Kit and application thereof in detecting vascular endothelial growth factors in anterior chamber fluid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] (1) Preparation of enzyme reactants

[0073] (a) Antibody treatment

[0074] PD10 column equilibration: Equilibrate the PD10 column with TSE85 buffer, 5 mL each time, repeat 5 times;

[0075] Antibody loading: Take the vascular endothelial growth factor-specific antibody for labeling (trade name 11066-R010, purchased from Sino Biological Company), the mass is 1 mg, the antibody concentration is 1 mg / mL, and the volume of the antibody needs to be 1000 μL; the antibody is added to the equilibrated For the PD10 column, add 1500 μL of TSE85 buffer;

[0076] Antibody recovery: Add 3mL of TSE85 buffer to the PD10 column and recover the antibody by gravity;

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Abstract

The invention relates to the technical field of aqueous humor antigen detection, in particular to a kit and application of the kit in detecting vascular endothelial growth factors in anterior chamberfluid; and the kit contains the following components which are independently stored: an enzyme reactant, a magnetic separation reagent, a stability enhancer, a calibrator, a cleaning concentrated solution and a substrate solution. The kit provided by the invention can accurately detect the content of the vascular endothelial growth factor in the anterior chamber fluid, has the advantages of smallsample demand, short detection time and wide linear range, and can obviously lower the background value when mutant alkaline phosphatase is used as a magnetic particle sealing agent, thereby loweringthe lowest detection limit of the reagent. The important application value is realized on the diagnosis of VEGF related ophthalmic diseases.

Description

technical field [0001] The invention relates to the technical field of aqueous humor antigen detection, in particular to a kit and its application in detecting vascular endothelial growth factor in anterior chamber fluid. Background technique [0002] In the field of ophthalmic diseases, although the precise pathogenesis of most ocular diseases is not completely clear, some key pathogenic molecules of important blinding diseases have been discovered in recent years, among which vascular endothelial growth factor (VEGF) is the most important. typical. [0003] Vascular endothelial growth factor (VEGF), also known as vascular permeability factor, is an important pro-angiogenic cytokine in the body. It plays a role in angiogenesis, endothelial cell growth, promoting cell migration and inhibiting apoptosis. [0004] Physiological concentration of VEGF is an essential cytokine for human development, injury repair and maintenance of normal physiological functions, but its overex...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6893G01N2800/16G01N2333/475
Inventor 夏超然仲惟星
Owner 杭州华视诺维医疗科技有限公司
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