Macrobrachium rosenbergii mitochondrial COI gene amplification primers

A technique for Macrobrachium rosenbergii and gene amplification, which is applied in the field of shrimp mitochondrial gene acquisition, can solve the problems of short COI gene fragments and limited data volume, and achieve the effect of single band and high amplification efficiency

Active Publication Date: 2020-08-25
HUZHOU TEACHERS COLLEGE
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Problems solved by technology

At present, there have been some research reports on the genetic diversity of the mitochondrial COI gene of Macrobrachium rosenbergii, but the COI gene fragments used in the existing studies are relatively short, about 400bp-500bp in length, and the amount of data provided is limited

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  • Macrobrachium rosenbergii mitochondrial COI gene amplification primers

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Embodiment Construction

[0027] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0028] In the embodiment of the present invention, the design, synthesis and amplification of the mitochondrial COI gene of Macrobrachium rosenbergii are carried out through the following steps.

[0029] 1. Download the sequence. Log in to the National Center for Biotechnology Information (NCBI, http: / / www.ncbi.nlm.nih.gov / ), download the existing full sequence of the mitochondrial genome of Macrobrachium rosenbergii, and its Genbank accession numbers are AY659990 and NC006880, and locate where the location of the COI gene.

[0030] 2. Primer design and synthesis. The 2 M. rosenbergii mitochondrial genome sequences downl...

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Abstract

The invention discloses macrobrachium rosenbergii mitochondrial COI gene amplification primers. Sequences of the primers are as follows: the sequence of an upstream primer is shown in SEQ ID NO.1, namely, COIF: 5'-CAACGTAATTGTCACTGCCCACG-3'; and the sequence of a downstream primer is shown in SEQ ID NO.2, namely, COIR: 5'-GTATCTGTGATCTGCTGGTGGGA-3'. By means of the macrobrachium rosenbergii mitochondrial COI gene amplification primers, the length of an obtained macrobrachium rosenbergii mitochondrial COI gene segment can be increased to about 1300 bp, and the primers have the characters of a single band and high amplification efficiency so that the requirements of subsequent macrobrachium rosenbergii mitochondrial genome research and macrobrachium rosenbergii population genetic diversity research can be met better.

Description

technical field [0001] The invention relates to the technical field of obtaining mitochondrial genes of shrimp organisms, [0002] In particular, the present invention relates to a primer for amplification of the Macrobrachium rosenbergii mitochondrial COI gene. Background technique [0003] Macrobrachium rosenbergii ( Macrobrachium rosenbergii ) belongs to Crustacea, Decapoda, Palaemonidae, Macrobrachium ( Macrobrachium ), native to the tropical and subtropical regions of Southeast Asia, is the largest variety in freshwater shrimp farming. In some natural waters of Southeast Asia, it was found that among the largest individuals, the male shrimp can reach 40cm in length and weigh 654g, and the female shrimp has a body length of 654g. It is 25cm long and weighs 200g. Mainland China introduced this shrimp in 1976. Through the efforts of many scientific researchers and industry promoters, China has become the country with the largest number of Macrobrachium rosenbergii farm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6806C12Q1/6888C12N15/11G16B25/20
CPCC12Q1/6806C12Q1/6888G16B25/20C12Q2600/156C12Q2531/113
Inventor 唐琼英杨国梁杨旻珉夏正龙谢巨洪蔡缪荧李景芬高权新
Owner HUZHOU TEACHERS COLLEGE
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