Method for co-extracting DNA and RNA of different samples

A co-extraction and sample technology, applied in the field of molecular biology, can solve the problems of inability to extract DNA and RNA, and achieve the effect of improving experimental safety and increasing accuracy.

Pending Publication Date: 2021-10-12
杭州圣庭医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] At present, there are many methods for extracting DNA and RNA separately in the market, but it is impossible to extract DNA and RNA together

Method used

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  • Method for co-extracting DNA and RNA of different samples
  • Method for co-extracting DNA and RNA of different samples
  • Method for co-extracting DNA and RNA of different samples

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Embodiment Construction

[0038] The present invention will be specifically introduced below in conjunction with the accompanying drawings and specific embodiments.

[0039] According to the following a kind of method of co-extracting DNA and RNA of different samples, the technical effect of the present invention is verified, comprising the steps:

[0040] Step 1, preparation and preparation of reagents:

[0041] 1. Lysate

[0042] 500mg / mL glycogen 200μL; 1M trisodium citrate dihydrate 550μL; 1M dithiothreitol (DTT) 550μL; 1M Tris-HCl (pH=8.0) 300μL; 10% TritonX-100 1mL; 0.5M EDTA 1mL; 10% N-lanroyl sarcdsine 5mL; 5M guanidine isosulfate 400μL mixed, stirred and mixed, and then dilute to 10mL. It should be noted that: this is only a preferred embodiment, as long as the lysate with guanidine isothiocyanate and dithiothreitol (DTT) is within the protection scope of the present invention.

[0043] 2.WB mother liquor

[0044] Mix 6 mL of 5M guanidine isosulfate; 100 μL of 1M Tris-HCl (pH=8.0); 400 μL ...

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Abstract

The invention discloses a method for co-extracting DNA (deoxyribonucleic acid) and RNA (ribonucleic acid) of different samples, and belongs to the field of molecular biology. The method comprises the following steps: step 1, liquefying the samples; step 2, adding enzyme into the liquefied sample; step 3, adding a lysate, and adding the supernatant in the step 2; the lysis solution comprising dithiothreitol and guanidine isosulfate; step 4, adding absolute ethyl alcohol, uniformly mixing, and adding magnetic beads; step 5, sucking and discarding supernate in the centrifugal tube; step 6, adding a washing solution into the centrifugal tube, uniformly mixing, and then sucking and discarding supernate; step 7, adding 80% ethanol into the centrifugal tube, uniformly mixing, and discarding supernate; and step 8, obtaining a DNA/RNA mixed solution. According to the method of the present invention, DNA/RNA can be extracted at the same time, pathogenic microorganisms are comprehensively obtained, infection sources are rapidly detected, and the accuracy of pathogenic recognition is improved.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a method for co-extracting DNA and RNA from different samples. Background technique [0002] The traditional detection method of clinical samples is the culture method, but this method takes a long time and has a low positive rate, which cannot meet the current needs. Molecular biology technology based on nucleic acid substances overcomes the limitations of culture technology and provides a powerful means for the detection of clinical pathogenic microorganisms. With the in-depth development of molecular biology technology, the diagnosis of diseases at the gene level has become a basic technical means of clinical diagnosis technology. [0003] But for the detection of clinical samples, it is necessary to detect a variety of pathogenic microorganisms for one sample, including not only DNA but also RNA, so it is very important to design a DNA / RNA co-extraction technology suitable f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10
CPCC12N15/1013
Inventor 谷红仓袁园园许佩松王云飞车仙荣
Owner 杭州圣庭医疗科技有限公司
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