USPIO-MOF assembly and preparation method and application thereof
An assembly and antibody technology, which is applied in the analysis, climate sustainability, water resources assessment, etc. using nuclear magnetic resonance, can solve the problem of inability to detect small molecules, etc. Effect of Transverse Relaxation Rate
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Embodiment 1
[0037]A USPIO-MOF assembly, comprising a USPIO material combined with a first antigen or a first antibody, a MOF material combined with a second antibody or a second antigen, the first antigen and the second antibody or the first antibody and the second antibody Through specific binding, the USPIO material is assembled on the surface of the MOF material to form a USPIO-MOF assembly.
[0038] The preparation method of this USPIO-MOF assembly comprises the following steps:
[0039] S1. Combining the USPIO material with the first antigen or the first antibody;
[0040] S2. Combining the MOF material with a second antibody or a second antigen;
[0041] S3. The USPIO material combined with the first antigen or the first antibody and the MOF material combined with the second antibody or the second antigen are formed into an assembly through antigen-antibody specific binding.
[0042] More specifically, the preparation method of the USPIO-MOF assembly of this embodiment includes th...
Embodiment 2
[0063] The USPIO-MOF assembly prepared in Example 1 was used as a magnetic relaxation switch sensor to detect bisphenol A.
[0064] Bisphenol A (BPA) is an important raw material widely used in the production of baby feeding bottles, dental fillings and the lining of food jars. The residues of BPA in food mainly come from food raw materials and food packaging materials. Considered an endocrine-disrupting chemical, numerous studies have shown that BPA may contribute to reproductive disorders including neurological and behavioral changes in infants and children, diabetes, hyperactivity, obesity and various cancers such as Breast, prostate and testicular cancer. my country's "Drinking Water Hygienic Standard" GB5749-2006 stipulates that the limit of BPA is 0.01mg / L. Specific migration or maximum residue shall not exceed 0.6mg / kg. We construct a magnetic relaxation switch sensor based on USPIO and MOF for the detection of BPA.
[0065] (1) Establishment of BPA standard curve
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