Low-temperature-resistant molecular marker A257 of penaeus japonicus and application of molecular marker A257

A technology of molecular markers and low temperature resistance, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of lack of molecular markers, and achieve the effect of accurate and reliable methods, increased quantity, and simple operation

Active Publication Date: 2021-11-09
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few studies on the development of molecular markers for low temperature tolerance of Penaeus japonicus, and there is a lack of markers that can be applied to molecular marker-assisted breeding in the industry

Method used

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  • Low-temperature-resistant molecular marker A257 of penaeus japonicus and application of molecular marker A257
  • Low-temperature-resistant molecular marker A257 of penaeus japonicus and application of molecular marker A257
  • Low-temperature-resistant molecular marker A257 of penaeus japonicus and application of molecular marker A257

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Experimental program
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Embodiment 1

[0026] 1. Screening of candidate molecular markers related to low temperature resistance

[0027] 1. Sequencing data filtering and comparison

[0028] Genomic DNA of Penaeus japonicus was extracted, 20 individuals in the low temperature tolerance group and 20 intolerant groups. Genomic DNA of Penaeus japonicus was extracted by using a centrifugal adsorption column that can specifically bind DNA and a unique buffer system. First, put about 30 mg of tissue samples into a 1.5 mL sterile enzyme centrifuge tube, add 200 μL of GA buffer and 20 μL of Proteinase K solution, vortex to mix, and incubate at 56°C until the tissue is completely lysed. Then add 200 μL of GB buffer, mix thoroughly by inversion, place at 70°C for 10 minutes, the solution becomes clear, cut it short and centrifuge to remove water droplets on the inner wall. Add 200 μL of absolute ethanol, invert and mix thoroughly. At this time, flocculent precipitates may appear, centrifuge briefly, and then add the resulti...

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Abstract

The invention provides a low-temperature-resistant molecular marker A257 of penaeus japonicus and application of the low-temperature-resistant molecular marker A257. The nucleotide sequence of the molecular marker A257 is shown as SEQ ID No.1, the molecular marker A257 is an Indel marker, and the low-temperature tolerance genotype is an AA homozygous insertion genotype. The nucleotide sequences of an amplification primer of the molecular marker A257 are as shown in SEQ ID No. 2 and SEQ ID No. 3. The molecular marker A257 provided by the invention is not limited by the growth stage of penaeus japonicus, the breeding process of the penaeus japonicus is obviously accelerated, a new variety of the penaeus japonicus with excellent low-temperature-resistant character is rapidly bred, and the detection of the low-temperature-resistant character of the penaeus japonicus by using the molecular marker A257 and the amplification primer thereof is accurate, reliable and simple to operate; the molecular marker is beneficial to healthy culture of penaeus japonicus and has wide application prospects.

Description

technical field [0001] The invention belongs to the field of molecular marker assisted breeding of aquatic animals, and in particular relates to a low temperature resistant molecular marker A257 of Penaeus japonicus and its application. Background technique [0002] Japanese prawns ( Marsupenaeus japonicus ), formerly known as Penaeus japonicus, belongs to the order Decapod, Penaeidae, and Penaeus spp. It is distributed in the tropical Indo-western Pacific Ocean, the east coast of Africa, Malaysia, Japan, North Korea, and the southeast coast of China. It is one of the main shrimp farming species in China. Penaeus japonicus grows rapidly, is resistant to dry dew, has bright color and high economic value, and is very popular in the aquatic product market and farmers. It is cultivated in various provinces and cities from the south of my country to the northern coastal areas, and has formed a certain scale. Breeding of improved species is of great significance to the Japanese sh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2531/113C12Q2565/125Y02A40/81
Inventor 任宪云贾少婷王琼孟宪亮邵慧鑫徐垚刘萍李健
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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