Large-scale plasma lipid targeted quantitative analysis method based on liquid chromatography-mass spectrometry

Abstract

According to the method, the external standard curve corrected by the internal standard is adopted for quantitative analysis of the concentration of the lipid, so that the defects that the deviation of pretreatment and mass spectrometry in the experiment process cannot be corrected and the actual lipid concentration change relationship cannot be accurately reflected in a pure peak area relative quantitative method are overcome; meanwhile, the problem that the nonlinear signal change of actual lipid on a mass spectrometer cannot be solved in the quantification process of an internal standard method of a single internal standard or multiple internal standards is also solved; the methyl tert-butyl ether-methanol extracting solution is adopted for extraction, pretreatment is carried out in the mode that isopropanol and acetonitrile are redissolved according to the ratio of 1: 1, operation is easy and rapid, solvent toxicity is small, and the method can be suitable for large-scale sample analysis.

Description

technical field [0001] The invention relates to analytical chemistry and biological detection, and is a large-scale plasma lipid quantitative analysis method based on liquid chromatography-mass spectrometry. Background technique [0002] Lipid is a class of insoluble small molecular substances, mainly divided into fatty acids, glycerolipids, phospholipids or cholesterol. Lipids are widely distributed in human cells and blood. Lipids are components of cell membranes and play an important role in energy storage and signaling molecules in the body. In human blood, lipid accounts for more than 65% of all substances and is an important component of blood. Due to the convenience of blood collection, it is the most common sample source in lipid research. At present, the lipid analysis of human blood is a research hotspot in the screening of cancer research markers, the body's metabolic mechanism and clinical diagnosis. [0003] The current lipid analysis mainly relies on liquid c...

AI Technical Summary

Problems solved by technology

For relative quantification, the quantitative results are inaccurate due to the inability to correct the deviation of pre-processing and mass spectrometry analysis in the experimental process.

Similarly, the absolute quantitative method of calculating the lipid concentration by internal standard correction cannot solve the problem of nonlinear signal changes and quantitative results of lipids on the mass spectrometer during the actual detection process.

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