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Rapid and efficient pepper transgenic method

A technology of transgenic and Zanthoxylum bungeanum, applied in genetic engineering, botany equipment and methods, biochemical equipment and methods, etc., can solve the problems of inability to improve traits, hybridization isolation, low efficiency, etc., and achieve great scientific research value, rapid cultivation, and reliable highly reliable effect

Active Publication Date: 2021-11-19
NORTHWEST A & F UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] The cultivation of new varieties by traditional breeding technology is limited by the isolation of intraspecific and interspecific hybridization, and many important traits cannot be improved by conventional breeding methods
Moreover, traditional breeding techniques are time-consuming and inefficient

Method used

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  • Rapid and efficient pepper transgenic method
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Embodiment Construction

[0040] The technical solutions of the present invention will be further described below through the accompanying drawings and embodiments.

[0041] As shown in the figure, a fast and efficient method for transgenic Zanthoxylum bungeanum, high-efficiency genetic transformation of Zanthoxylum bungeanum comprises the following steps:

[0042] (1) Plant material preparation

[0043] Sterilize the explants of Zanthoxylum bungeanum leaves, cut them into small pieces of 0.5cm×0.5cm, and pre-cultivate them for 2 days;

[0044] (2) Infection bacteria preparation

[0045] Pick a single clone on the LB medium supplemented with kanamycin and shake it overnight in 10ml LB medium, then draw 500μL of the above bacteria solution and add 50mL LB resistance medium to shake overnight;

[0046] Then centrifuge at 5000r / min for 5min to collect the bacteria, then resuspend the bacteria with 50mL of M20 medium, and control the OD value between 0.4-0.6;

[0047] Add kanamycin 500mg / L, rifampicin 2...

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Abstract

The invention discloses a rapid and efficient pepper transgenosis method. The efficient genetic transformation of pepper transgenosis comprises the following steps: (1) plant material preparation; (2) infected thalli preparation; (3) infection; (4) prolonged culture; (5) screening and culturing; (6) positive buds identifying; and (7) transgenic seedling formation. By adopting the quick and efficient pepper transgenosis method, specific excellent characters can be effectively transferred, and a new pepper variety can be quickly and efficiently bred.

Description

technical field [0001] The invention relates to the technical field of plant genetic engineering, in particular to a fast and efficient transgenic method for Zanthoxylum bungeanum. Background technique [0002] Zanthoxylum bungeanum belongs to the Rutaceae Zanthoxylum genus, which is resistant to drought and barrenness, with well-developed root system and strong adaptability. Because of its extremely high economic value and ecological value, it is considered to be one of the main ecological greening tree species in my country. In addition to ecological value, Zanthoxylum bungeanum is also an important condiment, spice and woody oil tree species in China, with high utilization value. In production practice, people need to improve the yield of Zanthoxylum bungeanum, reduce the difficulty of picking, increase the resistance of tree species and improve the quality, etc. It is also necessary to specifically change the content of certain substances in the processing of medicinal o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82A01H5/00A01H6/78C12Q1/6895
CPCC12N15/8202C12Q1/6895
Inventor 费希同魏安智王姝洁
Owner NORTHWEST A & F UNIV
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