Method for obtaining switchgrass transgene plant

A technology of transgenic plants, applied in the biological field, can solve the problems of inconsistency between regenerable cell parts and transformation competent cells, general application limitations of embryogenic callus, strong dependence on tissue culture technology, etc., to shorten the transformation cycle, transform Effect of improved genotype range, simplified procedure
CN101144091AInactive Publication Date: 2008-03-19NORTHWEST A & F UNIV
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Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
NORTHWEST A & F UNIV
Publication Date
2008-03-19
Estimated Expiration
Not applicable Β· inactive patent
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Abstract

The present invention discloses a method that a switchgrass transgenic plant is obtained by adopting agrobacterium-mediated transformation. When the genetic transformation of the switchgrass is operated, a seed which burgeons after being sterilized is used as a transformation acceptor, and the agrobacterium transformation and the co-culture are adopted; by screening on culture mediums of SMBP1, SMBP2 and SMBP3 in sequence, a resistant bud is obtained; the resistant bud is cultivated into a seedling in a rooting culture medium SMO; the seedling is transplanted to a greenhouse or the field to perform the detection of the molecular biological method, and a transgenic plant containing the exogenous aim is obtained by screening according to the molecular biological method and the target trait. The transgenic method of the switchgrass established by the present invention overcomes the disadvantage that the dependance of the agrobacterium-mediated transformation to the tissue culture condition is strong, widens the genotype range of the genetic transformation, and simplifies the transformation process, to provides a new approach for the genetic improvement of the switchgrass by adopting the transgenic technology.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, relates to the gene transformation of bioenergy plant switchgrass, and further relates to a method for obtaining switchgrass transgenic plants by transformation through the transformation method mediated by Agrobacterium. Background technique

[0002] Using transgenic technology to genetically improve energy plants is a new breakthrough in the application of modern genetic engineering technology in the field of agriculture. Switchgrass is an important new bioenergy plant and has become one of the main targets of genetic transformation. Among them, the use of transgenic technology to enhance the stress resistance of switchgrass, improve photosynthetic efficiency, increase biological yield, reduce lignin content and increase ethanol yield has become a research hotspot and shows good application prospects.

[0003] The Agrobacterium-mediated gene transformation system has become the preferred method fo...

Claims

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