Method for evaluating developmental toxicity of environmental pollutant H2S by using zebra fish

A technology of environmental pollutants and zebrafish, applied in the field of toxicology detection, can solve the problems of inability to assess or predict the ecosystem, and single-analyze the concentration of various substances, etc., to achieve the effect of short cycle, simple and convenient operation, and improved accuracy

Pending Publication Date: 2021-11-26
WENZHOU UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Water pollution is a serious threat to people's physical and mental health, and it is also a top priority for governments around the world to strictly control and manage it. Biohazard assessment of water quality to understand the degree of polluti...

Method used

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  • Method for evaluating developmental toxicity of environmental pollutant H2S by using zebra fish
  • Method for evaluating developmental toxicity of environmental pollutant H2S by using zebra fish
  • Method for evaluating developmental toxicity of environmental pollutant H2S by using zebra fish

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Embodiment 1

[0027] Example 1: A method for evaluating environmental pollutants H using zebrafish 2 The method for S developmental toxicity, its specific operation steps are as follows:

[0028] S1: use at least 5 pairs of male and female wild zebrafish (AB type) to mate and lay eggs, and collect fertilized eggs;

[0029] S2: Put the fertilized eggs into the wells of a 6-well plate containing 10 ml of zebrafish embryo culture medium, 30 eggs per well, and each 3 wells is a group of parallel repetitions, and divide the fertilized eggs into an experimental group and a control group;

[0030] S3: The control group was cultured normally, and the experimental group was grouped again. After grouping, 0.025 μM, 0.05 μM, 0.1 μM, 0.2 μM, 0.3 μM, 0.4 μM, 0.5 μM, 0.6 μM, 0.7 μM, 0.8 μM, 0.9 μM were added , 1.0 μM, 1.5 μM, and 2.0 μM NaHS solutions. During the subsequent culture process, the experimental group replaced the zebrafish embryo culture medium every day and added NaHS solution again; durin...

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Abstract

The invention discloses a method for evaluating developmental toxicity of an environmental pollutant H2S by using zebrafish, which comprises the following steps of: culturing zebrafish fertilized eggs in H2S environments with different concentrations, performing statistics on hatching of the zebrafish fertilized eggs by H2S with different concentrations and survival rate of juvenile fishes of the zebrafish fertilized eggs, and observing forms such as spines, postures and swimming bladders of the juvenile fishes, so as to evaluate the developmental toxicity of the environmental pollutant H2S. The method for evaluating the developmental toxicity of the environmental pollutant H2S by using the zebrafish is simple and convenient to operate, short in evaluation period, efficient and intuitive. The evaluation period can be completed within 10 days, the operation is simple, convenient, rapid and efficient, and the cost can be greatly reduced; the evaluation result of the evaluation method provided by the invention not only can provide a certain reference for the developmental toxicity of the environmental pollutant H2S in the water body to other water body organisms, but also can provide a certain data support and reference for the control of H2S in the water body.

Description

technical field [0001] The invention relates to the technical field of toxicology detection, in particular to a method for evaluating environmental pollutant H by using zebrafish. 2 S Methods of Developmental Toxicity. Background technique [0002] With the continuous development of human industrialization, a large amount of nutrients such as nitrogen and phosphorus enter lakes, rivers, bays and other slow-flowing water bodies, causing algae and other plankton to multiply rapidly, and the dissolved oxygen in the water body decreases rapidly, resulting in some aquatic organisms with high oxygen consumption. Animals die, and under the fermentation of anaerobic microorganisms, a large amount of H produced by the decomposition of these animal carcasses 2 S causes extremely serious harm to other aquatic animals, further deteriorating the water quality. [0003] Water pollution is a serious threat to people's physical and mental health, and it is also a top priority for governme...

Claims

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Application Information

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IPC IPC(8): G01N21/84
CPCG01N21/84
Inventor 彭仁义金立波李瑶琦嵇豪林苏娥孙达
Owner WENZHOU UNIVERSITY
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