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In-vitro preservation solution for siniperca chuatsi ovum and application of preservation solution

A technology for preserving liquid and mandarin fish eggs, applied in the biological field, can solve the problems of less ovarian fluid in fish, asynchronous sperm production in male fish, and limited acquisition, and achieve the effects of ensuring fertilization ability, solving technical problems, and ensuring vitality.

Pending Publication Date: 2022-01-04
WUHAN ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Eggs are relatively stable in ovarian fluid, but there is less ovarian fluid in fish, and the amount obtained is limited
During the process of artificial induction of mandarin fish, the problem of asynchronous spawning of female fish and male fish often occurs. How to maintain the vigor of eggs in a short period of time and maintain a high fertilization rate and hatching rate of eggs during in vitro operation is the key to artificial reproduction of mandarin fish. A practical problem that must be solved in

Method used

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  • In-vitro preservation solution for siniperca chuatsi ovum and application of preservation solution
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  • In-vitro preservation solution for siniperca chuatsi ovum and application of preservation solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: the preparation of mandarin fish ovum preservation solution in vitro

[0022] An in vitro preservation solution for mandarin fish eggs, the formula of which comprises the following components and their volume fractions: 60%-70% of culture solution, 15%-20% of nutrient solution, 9%-23% of fish ovary solution and 1% of antibacterial solution ~2%.

[0023] Among them, the formula of the culture solution: glycine 500-600mg / L, glucose 1000-2000mg / L, HEPES800-1200mg / L, sodium dihydrogen phosphate 200-400mg / L, potassium chloride 170-380mg / L, magnesium sulfate 200-230mg / L, sodium chloride 5000~7250mg / L, calcium chloride 110~130mg / L, sodium bicarbonate 2000~2500mg / L.

[0024] Wherein, the nutrient solution includes the following components and their volume fractions: 40% of bovine serum albumin and 60% of fish serum.

[0025] Among them, bovine serum albumin formula: bovine serum albumin 500-1000mg / L.

[0026] The fish serum is obtained by the following method:...

Embodiment 2

[0030] Embodiment 2: each component and its volume fraction of the best preservation solution

[0031] According to the different proportions of culture solution, nutrient solution, fish ovary solution and antibacterial solution, three mixing experiments with different volume fractions were designed. Experimental group 1 included the following components and their volume fractions: culture solution 60%, nutrient solution 15% %, fish ovary liquid 23% and antibacterial liquid 2%, the experimental group two contains the following components and their volume fractions: culture liquid 65%, nutrient liquid 17.5%, fish ovary liquid 16% and antibacterial liquid 1.5%, experimental group three It contains the following components and their volume fractions: 70% of culture fluid, 20% of nutrient fluid, 9% of fish ovary fluid and 1% of antibacterial fluid.

[0032] Among them, the formula of the culture solution: glycine 550mg / L, glucose 1500mg / L, HEPES1000mg / L, sodium dihydrogen phosphat...

Embodiment 3

[0042] Embodiment 3: in vitro preservation of mandarin fish ovum

[0043] The application of the optimum volume fraction mandarin ovum preservation solution in vitro of embodiment 2 adopts the following steps: after mixing the culture solution 6.5ml of embodiment 2, nutrient solution 1.75ml, fish ovary fluid 1.6ml and antibacterial solution 0.15ml, use Sodium dihydrogen phosphate to adjust the pH to 8.00, and prepare it for use now. Soak the collected mature mandarin mandarin eggs in a glass dish containing egg preservation solution in vitro (1 ml / 200 capsules), and soak for 2 hours at 16-20°C Afterwards, the preservation solution was sucked off to carry out the artificial insemination operation, and the results are shown in Table 2:

[0044] Table 2 Effects of different treatments on fertilization rate, hatching rate and deformity rate of mandarin fish eggs

[0045]

[0046] As can be seen from Table 2, the in vitro preservation solution of a kind of mandarin ovum that the ...

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Abstract

The invention discloses an in-vitro preservation solution for siniperca chuatsi ovum and application of the in-vitro preservation solution. The preservation solution is prepared from, by volume, 60%-70% of culture solution, 15%-20% of nutrient solution, 9%-23% of fish ovary solution and 1%-2% of antibacterial solution. The invention further discloses application of the in-vitro preservation solution for the siniperca chuatsi ovum, in-vitro mature siniperca chuatsi ovum is soaked in the in-vitro preservation solution for the siniperca chuatsi ovum under the soaking conditions that each 150-200 siniperca chuatsi ovum is 0.8-1 ml, and after the siniperca chuatsi ovum can be soaked and preserved for 2-3 hours at the temperature of 16-20 DEG C, the fertilization rate is 90% or above, the hatching rate is 89% or above, and the malformation rate is 0.5% or below. The technical problem caused by desynchrony of female and male spawning induction effect time of the siniperca chuatsi is solved.

Description

technical field [0001] The invention provides an in vitro preservation solution of mandarin fish eggs and application thereof, belonging to the field of biotechnology. Background technique [0002] With the gradual reduction of mandarin fish resources in my country, the genetic improvement of mandarin fish has been widely carried out in various places. Due to the large difference in the time of sexual maturity of mandarin fish, the hybridization work has higher requirements on the maturity of the gonads. Overripe or not fully mature will seriously affect the fertilization rate of hybrid breeding. [0003] Fish mainly reproduce by in vitro fertilization and in vitro development. The ovaries of teleosts are closed ovaries, which are surrounded by the ovarian membrane formed by the ovarian peritoneum. Mature eggs fall in the ovarian cavity instead of the abdominal cavity, and are exported out of the body through the oviduct. Fish eggs undergo morphological and biochemical cha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 宋文曾可为王守荣
Owner WUHAN ACADEMY OF AGRI SCI
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