Method for detecting residual quantity of antituberculous drugs in beef and mutton

Abstract

The invention discloses a method for detecting the residual quantity of antituberculous drugs in beef and mutton, and relates to the technical field of beef and mutton detection. The method comprises the following steps of: S1, extracting beef and mutton tissue soaking liquid; according to the method for detecting the residual quantity of the antituberculous drugs in beef and mutton, dinitrophenylhydrazine and streptomycin react in an acid solution to generate yellow color, and the detected absorbance value is compared with an absorbance standard curve, so that the residual quantity of the antituberculous drugs is obtained. According to the method, the existence and size of an inhibition zone on a reagent plate containing bacillus subtilis liquid in a test box are observed, the residual quantity of the antituberculous drugs is obtained by contrasting an observation result with an inhibition zone standard curve, a standard numerical value is analyzed and obtained according to the two obtained numerical values, and if the difference between the two numerical values is large, an experiment is performed again, so that the accuracy of the detection method is improved, and deviation of a detection result caused by detection by using a single detection method is prevented, so that food safety is guaranteed.

Description

Technical field [0001] The present invention relates to the technical field of detection of beef and mutton, particularly to a method for detecting residual amount of beef and sheep anti-tuberculosis drugs. Background technique [0002] Sheep Tuberculosis is a chronic infectious disease caused by Mycobacterium tuberculosis. Granuloma formation and pathological features caseous necrosis or calcified nodules in various organs. Clinically frequency cough, dyspnea, and is characterized by the body surface lymph nodes. Is dairy cattle, sheep because of its great harm to human milk, breeding stock should be eliminated. People, cattle, poultry may be the disease. Livestock to cattle (especially dairy cows) most susceptible. Sick animals are the source of infection, mainly through the respiratory tract infection, but also through the digestive tract (seen in calves). Antituberculosis drugs more commonly used drugs such as streptomycin, anti-tuberculosis drugs Residues sheep meat conventi...

AI Technical Summary

Problems solved by technology

Streptomycin and other drugs are commonly used in anti-tuberculosis drugs. The existing detection methods for anti-tuberculosis drug residues in beef and mutton food are not accurate, and there are large deviations in the detected antibiotic residues. The beef and mutton of anti-tuberculosis drugs will cause harm to the human body

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