Method for detecting residual quantity of antituberculous drugs in beef and mutton
A detection method and anti-tuberculosis technology, applied in the field of beef and mutton detection, can solve the problems of human injury, inaccurate detection method, deviation of antibiotic residue, etc., to achieve the effect of improving accuracy and ensuring food safety
Pending Publication Date: 2022-02-18
XINJIANG ACADEMY OF ANIMAL SCI QUALITY STANDARDS INST OF ANIMAL HUSBANDRY XINJIANG UYGUR AUTONOMOUS REGION SHEEP & WOOL CASHMERE QUALITY SAFETY SUPERVISION & INSPECTION CENT
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Problems solved by technology
Streptomycin and other drugs are commonly used in anti-tuberculosis drugs. The existing detection methods for anti-tuberculosis drug residues in beef and mutton food are not accurate, and there are large deviations in the detected antibiotic residues. The beef and mutton of anti-tuberculosis drugs will cause harm to the human body
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[0037] See Figure 1 to 5 The present invention provides a technical solution: A beef and sheep anti-tuberculosis drugs Residues detecting method, comprising the steps of:
[0038] S1: red meat extract tissue soaking liquor;
[0039] S2: The solution was dried and the dried solid was broken process;
[0040] S3: The resultant was poured in a test tube and the test cartridge is detected;
[0041] S4: the observed changes in the test tube cartridge, is determined in cattle and sheep anti-tuberculosis drug compliance.
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The invention discloses a method for detecting the residual quantity of antituberculous drugs in beef and mutton, and relates to the technical field of beef and mutton detection. The method comprises the following steps of: S1, extracting beef and mutton tissue soaking liquid; according to the method for detecting the residual quantity of the antituberculous drugs in beef and mutton, dinitrophenylhydrazine and streptomycin react in an acid solution to generate yellow color, and the detected absorbance value is compared with an absorbance standard curve, so that the residual quantity of the antituberculous drugs is obtained. According to the method, the existence and size of an inhibition zone on a reagent plate containing bacillus subtilis liquid in a test box are observed, the residual quantity of the antituberculous drugs is obtained by contrasting an observation result with an inhibition zone standard curve, a standard numerical value is analyzed and obtained according to the two obtained numerical values, and if the difference between the two numerical values is large, an experiment is performed again, so that the accuracy of the detection method is improved, and deviation of a detection result caused by detection by using a single detection method is prevented, so that food safety is guaranteed.
Description
Technical field [0001] The present invention relates to the technical field of detection of beef and mutton, particularly to a method for detecting residual amount of beef and sheep anti-tuberculosis drugs. Background technique [0002] Sheep Tuberculosis is a chronic infectious disease caused by Mycobacterium tuberculosis. Granuloma formation and pathological features caseous necrosis or calcified nodules in various organs. Clinically frequency cough, dyspnea, and is characterized by the body surface lymph nodes. Is dairy cattle, sheep because of its great harm to human milk, breeding stock should be eliminated. People, cattle, poultry may be the disease. Livestock to cattle (especially dairy cows) most susceptible. Sick animals are the source of infection, mainly through the respiratory tract infection, but also through the digestive tract (seen in calves). Antituberculosis drugs more commonly used drugs such as streptomycin, anti-tuberculosis drugs Residues sheep meat conventi...
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IPC IPC(8): C12Q1/04G01N21/31G01N21/78
CPCC12Q1/04G01N21/78G01N21/31
Inventor 魏佩玲宫平胡波张雅惠许艳丽张蓉银王乐赛迪古丽·赛买提柴婷王晓涛于海龙
Owner XINJIANG ACADEMY OF ANIMAL SCI QUALITY STANDARDS INST OF ANIMAL HUSBANDRY XINJIANG UYGUR AUTONOMOUS REGION SHEEP & WOOL CASHMERE QUALITY SAFETY SUPERVISION & INSPECTION CENT
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