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Oyster protein source anti-skin photoaging octapeptide, and preparation method and application thereof

A chemical modification, amino acid technology, applied in the field of protein, to achieve the effect of promoting the formation of wrinkles

Pending Publication Date: 2022-02-25
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no research report on the anti-UVB-induced skin photoaging of Hong Kong oyster and its related products

Method used

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  • Oyster protein source anti-skin photoaging octapeptide, and preparation method and application thereof
  • Oyster protein source anti-skin photoaging octapeptide, and preparation method and application thereof
  • Oyster protein source anti-skin photoaging octapeptide, and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0087] Example 1 Preparation of oyster protein source anti-skin photoaging octapeptide

[0088] 1. Preparation of Oyster protein enzymatic hydrolysate (OPEH) and ultrafiltration components

[0089] Extraction of oyster protein: fresh Hong Kong oyster meat→washing and weighing→preliminary beating→adding distilled water according to the material ratio of 1:3 (100g oyster meat: 300mL distilled water)→0.2mol / L NaOH to adjust the pH to 12.0→high-speed homogenizer homogenization 5min (ice bath)→stir in a chromatographic cabinet at 4°C for 3h→centrifuge (10000r / min, 4°C, 20min)→take the supernatant→adjust the pH of the supernatant to 4.8 with 0.2mol / L HCl→centrifuge at 4°C for 20min (10000r / min, 4°C, 20min) → take the precipitate, and freeze-dry it to obtain oyster protein.

[0090] Enzymatic hydrolysis of oyster protein: weigh oyster protein → add distilled water according to the material ratio of 1:3 (100g oyster meat: 300mL distilled water) → adjust the pH of the sample solution...

Embodiment 2

[0153] Embodiment 2 Anti-photoaging activity verification of synthetic polypeptide

[0154] Adopt CCK-8 method to measure HaCaT cell viability (method is the same as the protective effect of P1~P6 on the HaCaT cell viability of UVB aging in the embodiment 1, difference is only that sample final concentration is 20 μ M), the result is as follows Figure 23 Shown: After UVB irradiation, the cell viability of HaCaT cells decreased to 55.61% of that of the CT group, and the synthetic polypeptide RKNEVLGK and WNLNP at a final concentration of 20 μM can significantly improve the viability of HaCaT cells aged by UVB, reducing the cell viability of HaCaT cells Respectively increased to 77.86% and 84.08% of the CT group.

[0155] Adopt Western Blotting method to measure the inhibition of MMP-1 expression in the HaCaT cells of UVB aging (method is the same as the inhibition of MMP-1 expression in P1~P6 in the HaCaT cells in the embodiment 1, the difference is only that the sample final ...

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Abstract

The invention belongs to the technical field of protein, and discloses an oyster protein source anti-skin photoaging octapeptide, and a preparation method and application thereof. The amino acid sequence of the oyster protein source anti-skin photoaging octapeptide is as follows: a) RKNEVLGK (SEQ ID NO.3); or b) an amino acid sequence which is obtained by substitution or deletion of one or more amino acids of the amino acid sequence as shown in SEQ ID NO.3 and has the same or similar functions. The octapeptide has antioxidant activity, can promote type I collagen precursor secretion, can inhibit MMP-1 expression, has a protection effect on keratinocytes, and can be used for preparing products for resisting skin photoaging or aging, preventing or repairing keratinocyte light damage caused by UVB, and preventing or improving skin damage caused by UVB radiation.

Description

technical field [0001] The invention belongs to the technical field of protein, and in particular relates to an oyster protein source anti-skin photoaging octapeptide, a preparation method and application thereof. Background technique [0002] In recent years, due to the destruction of the ozone layer, more ultraviolet rays (Ultraviolet light, UV) radiate to the earth's surface through the ozone layer, making the number of patients suffering from skin diseases increase year by year, seriously endangering the physical and mental health of the people. Skin photoaging is a premature aging of the skin caused by the continuous accumulation of excessive ultraviolet radiation. The ultraviolet rays radiated to the ground can be divided into three types according to their wavelengths: UVA (320-420nm); UVB (275-320nm) and UVC (200-275nm), among which UVB can pass through the epidermis and directly reach the epidermis and dermis The interface is the main cause of skin photoaging. Ski...

Claims

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Application Information

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IPC IPC(8): C07K7/06C07K1/107C12N15/12A61K38/08A61P17/16A61P17/18A61K8/64A61Q17/04A61Q19/08A61Q19/00
CPCC07K7/06A61P17/16A61P17/18A61K8/64A61Q17/04A61Q19/08A61Q19/004A61K38/00
Inventor 彭志兰章超桦秦小明曹文红高加龙郑慧娜卢虹玉林海生
Owner GUANGDONG OCEAN UNIVERSITY
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