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Preparation method of prp serum for repairing sport injury

A technology for sports injury and serum preparation, which is applied in the preparation methods of peptides, chemical instruments and methods, and derived from serum immunoglobulins, etc. It can solve the problems of poor repeatability, inability to conduct research on the efficacy of prp serum, cumbersome and other problems, and achieve rapid promotion. repaired effect

Pending Publication Date: 2022-03-01
南京国青血液净化科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the actual operation process, it is cumbersome and has poor reproducibility, and it is impossible to conduct research on the efficacy of prp serum for sports injury repair, so as to obtain the best treatment concentration plan and further improve the repair efficiency of sports injuries

Method used

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  • Preparation method of prp serum for repairing sport injury
  • Preparation method of prp serum for repairing sport injury

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Comparison scheme
Effect test

Embodiment 1

[0026] Such as Figure 1-2 As shown, the preparation method of prp serum for sports injury repair described in this embodiment comprises the following steps:

[0027] Step 1: Mix the whole blood with the anticoagulant to obtain anticoagulated blood, open the one-way regulating valve 130 and the peristaltic pump 120, the anticoagulated blood enters from the whole blood delivery tube 110, and the peristaltic pump 120 promotes the anticoagulated blood from the whole blood Delivery pipe 110 enters in nanofiltration centrifuge box 210; Wherein, anticoagulant is selected from the sodium citrate solution of 2.5wt%, adds the sodium citrate solution 10mL of 2.5wt% in every 100mL whole blood;

[0028] Step 2: The anticoagulated blood passes through the nanofiltration of the first nanofiltration membrane 223 to remove white blood cells and red blood cells in the whole blood, and the first centrifugal mechanism 240 centrifuges to obtain plasma and serum, and the plasma and serum pass thro...

Embodiment 2

[0034] Such as Figure 1-2 As shown, this embodiment provides a device for preparing prp serum for sports injury repair, including a whole blood delivery mechanism 100 for delivering anticoagulated blood formed after mixing whole blood with an anticoagulant; nanofiltration centrifugal mechanism 200 , used for secondary nanofiltration and centrifugation of anticoagulated blood to obtain different concentrations of plasma serum; prp serum mixing refrigeration mechanism 300, used for mixing pre-prepared platelet-rich plasma serum with different concentrations of plasma serum to obtain different concentrations prp serum and refrigerated.

[0035]In the device for preparing prp serum for repairing sports injuries in this embodiment, the anticoagulated blood formed by mixing whole blood and anticoagulant is transported through the whole blood delivery mechanism 100, and the anticoagulated blood is transported through the nanofiltration centrifugal mechanism 200. Plasma serum of dif...

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Abstract

The invention provides a preparation method of prp serum for repairing sport injury, and relates to the technical field of serum preparation. The preparation method of the prp serum for sports injury repair comprises the following steps: conveying anticoagulant blood formed by mixing whole blood and an anticoagulant, carrying out secondary nanofiltration and centrifugation on the anticoagulant blood to obtain plasma serums with different concentrations, mixing platelet-rich plasma serums prepared in advance with the plasma serums with different concentrations to obtain prp serums with different concentrations, and refrigerating to obtain the prp serum for sports injury repair. The prp serum can be mixed with sodium hyaluronate gel to be injected into the sport injury part, the environment of natural cartilage is fully simulated by means of the gel with high water content, and the prp serum is easy to degrade and non-toxic, so that the curative effect research of the prp serum for repairing the sport injury is facilitated, the optimal treatment concentration scheme is obtained, and the quick repair of the sport injury part is promoted.

Description

technical field [0001] The invention relates to the technical field of serum preparation, in particular to a method for preparing prp serum for sports injury repair. Background technique [0002] Platelet-rich plasma (platelet-rich plasma, PRP) is the plasma containing high concentration of platelets obtained by centrifuging animal or human whole blood. PRP contains a large number of growth factors, including platelet-derived growth factor, transforming growth factor-β, insulin-like growth factor, epithelial growth factor and vascular epithelial growth factor, etc., which can effectively promote wound healing. At present, PRP has been clinically applied in the treatment of chronic ulcer wounds, the treatment of diabetic foot ulcers, the treatment of burn wounds, and skin beauty, and has achieved obvious therapeutic and repair effects. In addition, there are clinical studies on the application of PRP in the treatment of fracture nonunion, nonunion, osteonecrosis, osteoarthri...

Claims

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Application Information

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IPC IPC(8): A61K35/16A61K9/06A61K47/36A61P19/04C07K1/14C07K1/34C07K16/06
CPCA61K35/16A61K9/0019A61K9/06A61K47/36A61P19/04C07K16/065C07K1/34C07K1/14
Inventor 邓彦玮
Owner 南京国青血液净化科技有限公司
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