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Application of the compound cyproheptadine hydrochloride in the preparation of drugs for preventing or treating African swine fever

A technology of cyproheptadine hydrochloride and African swine fever, applied in the field of prevention or treatment of African swine fever, the compound cyproheptadine hydrochloride, can solve the problems that no research shows that Cyproheptadinehydrochloride has anti-African swine fever virus effect, and prevents the virus from invading host cells , the effect of reducing transcription and protein expression levels

Active Publication Date: 2022-07-12
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Okuma Hirohisa and other researchers have reported this compound in academic articles (see Okuma Hirohisa, Iijima Kazuyuki, Yasuda Takashi et al. Preventive effect of cyproheptadine hydrochloride in refractory patients with frequent migraine. [J]. Springerplus, 2013, 2:573.) , but there is no research showing that Cyproheptadinehydrochloride has anti-ASF virus effect

Method used

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  • Application of the compound cyproheptadine hydrochloride in the preparation of drugs for preventing or treating African swine fever
  • Application of the compound cyproheptadine hydrochloride in the preparation of drugs for preventing or treating African swine fever
  • Application of the compound cyproheptadine hydrochloride in the preparation of drugs for preventing or treating African swine fever

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 The effect of compound Cyproheptadine hydrochloride on the replication of African swine fever virus and gene transcription and expression

[0038] 1. Observation of the effect of the compound Cyproheptadine hydrochloride on ASFV infection and replication under fluorescence

[0039] Porcine alveolar macrophages (PAM, 2 × 10) were cultured in RPMI 1640 + 10% FBS medium in 12-well plates 6 / well), the experimental group was treated with ASFV fluorescent virus (MOI=0.5) mixed with different concentrations of Cyproheptadinehydrochloride (12 μM, 18 μM, 22 μM, 50 μM) dissolved in DMSO (2 Fluorescence changes were observed under a microscope after 48h of conditioned culture.

[0040] The experimental results are as figure 1 shown, figure 1 A shows that there was no significant difference between the infected control group and the infected blank group under fluorescence and white light, indicating that DMSO had no effect on the infection and replication of cells.

...

Embodiment 2

[0068] Cytotoxicity of the compound Cyproheptadine hydrochloride of Example 2

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Abstract

The invention belongs to the technical field of African swine fever treatment, in particular to the application of the compound cyproheptadine hydrochloride in preventing or treating African swine fever. The present invention finds that the compound cyproheptadine hydrochloride can significantly inhibit the replication of ASFV. And found that cyproheptadine hydrochloride not only inhibits the transcription and protein expression of the target protein D1133L, but also reduces the transcription and protein expression levels of p30 and p72, preventing the virus from invading host cells, and can be used to inhibit the early and late infection of ASFV. Therefore, the compound cyproheptadine hydrochloride can be used to prevent or treat African swine fever.

Description

technical field [0001] The invention belongs to the technical field of African swine fever treatment, in particular to a new use of a compound Cyproheptadine hydrochloride for preventing or treating African swine fever. Background technique [0002] African swine fever (ASF) is an acute, highly contagious disease caused by African swine fever virus (ASFV) infection with a mortality rate as high as 100%. Since its introduction into China in 2018, it has caused significant economic losses to the domestic pig industry. There is currently no commercial vaccine, so the development of effective antiviral drugs at a reasonable cost is of great significance for the treatment of African swine fever. Although various classes of active anti-ASFV drugs have been reported, the in vivo efficacy of these compounds has not been evaluated. [0003] ASFV is a double-stranded DNA virus that replicates mainly in monocytes and macrophages and encodes more than 50 structural proteins and more t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/4418A61P31/20
CPCA61K31/4418A61P31/20Y02A50/30
Inventor 张克山郑海学崔卉梅田宏朱紫祥冯涛杨帆曹伟军马旭升茹毅郭建宏刘湘涛
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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