Method for creating high-combining ability glutinous sorghum sterile line Ji 5535A by hybridizing backbone japonica sorghum parent lines

A technology of backbone japonica and combining ability, applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve the problems of poor operability, high plant height of waxy materials, and difficulty in utilization.

Pending Publication Date: 2022-04-12
JILIN ACAD OF AGRI SCI
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  • Abstract
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Problems solved by technology

[0003] The technology currently used is the manual emasculation of waxy sorghum germplasm resources and waxy sorghum germplasm resources. 2 Dai Zhongjian selects waxy materials, but this method often selects waxy materials with high plant height and poor comprehensive traits, which are difficult to use in breeding; Journal "Rapid Identification Techniques and Applications of Waxy Characteristics of Sorghum Starch "Middle B 1 (WW)×WW Ww all the way to BC 4 f 1 , the operability is poor, and the intermediate materials on both sides have been separated in this process. Not only waxy traits are selected for breeding, but also traits such as plant height, plant type, and grain color are equally important; in the process of backcrossing, it itself been separating, not homozygous

Method used

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  • Method for creating high-combining ability glutinous sorghum sterile line Ji 5535A by hybridizing backbone japonica sorghum parent lines

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Embodiment

[0030] Such as figure 1 As shown, a kind of method that the present invention proposes utilizes backbone japonica sorghum parent line hybridization to create high combining ability glutinous sorghum CMS line Ji 5535A, comprising the following steps:

[0031] Step 1: In the first summer, the main maintainer line of japonica sorghum, Ji 2055B, was selected as the female parent, and the japonica sorghum maintainer line 521B was used as the male parent, and planted in the experimental field, and artificial emasculation was carried out to obtain F 1 substitute seeds;

[0032] Step 2: In the first winter, the F obtained in Step 1 will be 1 The generation seeds were planted in the base, and the uniform single plant was selected as the female parent, and the japonica sorghum maintainer line 521B was used as the male parent to backcross once to obtain BC 1 f 1 Substitute seed, the purpose of this step is to accelerate the appearance of homozygous waxy material;

[0033] Step 3: BC ...

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Abstract

The invention relates to the technical field of sorghum creation and cultivation, in particular to a method for creating a glutinous sorghum sterile line Ji 5535A with high combining ability by hybridizing backbone japonica sorghum parent lines. Aiming at the problems that the existing glutinous sorghum variety is not suitable for being planted in a spring sowing early-maturing area and the operability of cultivating the glutinous sorghum variety is poor, the following technical scheme is provided: 1, obtaining F1-generation seeds; 2, BC1F1 generation seeds are obtained; 3, obtaining BC1F2 generation seeds; 4, obtaining BC1F3 generation seeds; 5, obtaining BC1F4 generation maintainer line seeds; 6, obtaining F1 generation sterile line seeds and BC1F5 generation maintainer line seeds; 7, obtaining BC1 generation sterile line seeds and BC1F6 generation maintainer line seeds; 8, obtaining BC2 generation sterile line seeds and BC1F7 generation maintainer line seeds; 9, obtaining a BC3 generation sterile line and BC1F8 generation maintenance type seeds; 10, obtaining BC4 generation sterile line seeds and BC1F9 generation maintainer line seeds; and 11, naming Ji 5535A and Ji 5535B. The method is suitable for planting in a spring sowing early-maturing area, is a sterile line with excellent comprehensive characters such as plant height and quality, and is mainly applied to cultivation of the sterile line of glutinous sorghum.

Description

technical field [0001] The invention relates to the technical field of creation and cultivation of sorghum, in particular to a method for creating a waxy sorghum male sterile line Ji 5535A with high combining ability by crossing a backbone japonica sorghum parent line. Background technique [0002] Famous wineries in the south use glutinous sorghum as the raw material for winemaking, but most of the glutinous sorghum grown in the south is a local variety, and the output cannot meet the needs of the winery. The sorghum spring-sowing early-maturing areas in Northeast my country are the main production areas of sorghum in my country, and the japonica sorghum hybrids are mainly planted. Sell ​​waxy sorghum hybrid seeds suitable for spring-sowing late-maturing areas to farmers to develop contract agriculture. The purchase price of grain is relatively high. When the harvest is mature, it will be beaten by frost, causing huge yield losses to farmers; sorghum is a three-line hybrid...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01G22/20A01H1/04
Inventor 陈冰嬬王鼐石贵山于淼唐玉劼王江红刘红欣徐宁李洪奎黄炎李海青
Owner JILIN ACAD OF AGRI SCI
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