Seed production method for improving growth of pinctada martensii with golden shell color

A technology of Pinctada martensii and golden shell, which is applied in the field of genetic breeding technology of aquatic animals and aquaculture, can solve problems such as the separation of shell color traits, and achieve the effect of improving growth and not reducing the purity of shell color

Active Publication Date: 2022-04-15
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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Problems solved by technology

[0006]In breeding, the hybridization method can usually be used to improve growth, but the shell color traits will be separated, and it is impossible to simultaneously ensure that the shell color purity is not reduced. enhance its growth performance

Method used

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  • Seed production method for improving growth of pinctada martensii with golden shell color

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Embodiment

[0019] Step 1: In May 2019, select female individuals with relatively full gonads from the No. 50 population of Pinctada martensii (this group is relatively large, derived from the first-generation selection breeding population of Zhanjiang breeding population, and the shell height is about 5cm) 3 Three male individuals were selected from the long-eared pearl oysters obtained from fishing (from Beihai, Guangxi, with a shell height of about 7 cm), and the eggs and sperm were dissected and stored in a beaker equipped with filtered seawater. Filter through a sieve to remove impurities and tissue fragments. The eggs were quickly added to flat-bottomed glass containers filled with ammonia seawater of 0.006%, 0.009% and 0.012% by volume respectively, and stayed for 10 minutes. Then, an appropriate amount of sperm was added and mixed evenly to allow fertilization. Three gradients (23°C, 26°C and 29°C) were set for fertilization and development water temperature. Change the water twi...

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Abstract

The invention discloses a seed production method for improving growth of pinctada martensii with a golden shell color. The method comprises the following steps: (1) selecting female individuals as female parents from a pinctada martensii population with a rapid growth characteristic, performing artificial hybridization by taking pinctada martensii male individuals as male parents, and expanding the population number through self-reproduction in the population; and (2) selecting female individuals with mature gonads from the propagation expanding population obtained in the step (1) as female parents, selecting male individuals with yellow shells from the golden shell color Pinctada martensii strains as male parents, and carrying out artificial fertilization, cultivation and breeding to obtain the improved golden shell color Pinctada martensii. The method can improve the size of the yellow shell individual, can guarantee the shell color purity, and is convenient for production and application.

Description

Technical field: [0001] The invention belongs to the field of aquatic animal genetic breeding technology and aquaculture, and in particular relates to a seed production method for improving the growth of golden-yellow shell-colored Pinctada martensii. Background technique: [0002] Studies have shown that there is a certain correlation between the individual's choice of shell color in the growth process and its growth environment (including the temperature and salinity of the water area, etc.), geographical location and physiological factors. According to some shellfish research reports, shell color polymorphism is a simple Mendelian inheritance, but there is no clear conclusion whether the selection of shell color is necessarily related to growth traits, physiological traits and some economic traits. [0003] Pinctada martensii Dunker (Pinctada martensii Dunker) belongs to Bivalvia, Pearl Oysteraceae, and Pinctada genus. It is an important cultured seawater pearl shellfish....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/54
CPCA01K61/54Y02A40/81
Inventor 何毛贤张华刘文广
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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