CadR gene mutant, recombinant vector containing mutant and application of recombinant vector

Abstract

The invention discloses a CadR gene mutant which has a nucleotide sequence as shown in SEQ ID NO: 6 or a nucleotide sequence complementary with the nucleotide sequence as shown in SEQ ID NO: 6. The CadR gene mutant disclosed by the invention has higher response sensitivity and specificity to cadmium, a recombinant vector containing the CadR gene mutant and an engineering bacterium of the recombinant vector serving as a cadmium-induced biosensor can improve the detection sensitivity, meanwhile, the influence of other heavy metals on sensor detection is weakened, and cadmium within a safety standard range can be detected; as an expression vector of the recombinant protein, a trace amount of cadmium can be used as an inducer to induce the expression of the target protein; the gene has application value in the aspects of cadmium pollution monitoring and protein expression regulation.

Description

technical field [0001] The invention relates to the technical field of biosensing and environmental pollutant detection, in particular to a CadR gene mutant, a recombinant vector containing the mutant and an application thereof. Background technique [0002] As an important environmental risk factor, cadmium pollution has been widely concerned because it can quickly accumulate in soil and water, and eventually enter the human body through the food chain and cause serious health hazards to the human body. Therefore, the detection of cadmium in the environment is particularly important and is regarded as a standard to measure the degree of environmental pollution. The existing traditional physical and chemical methods such as atomic absorption spectroscopy (AAS) and inductively coupled plasma mass spectrometry (ICP-MS) have limited sample processing. The process is complicated, the detection cost is high, and harmful by-products are easily produced. [0003] Bacteria are idea...

AI Technical Summary

Problems solved by technology

In addition, since the prokaryotic expression system of E.coli is currently the most widely used in the expression of recombinant proteins, the expression speed of this system is too fast, the incorrect folding of the peptide chain and the hydrophobicity of the polypeptide chain exist in the process of expressing the recombinant protein. There are problems such as interaction between regions, which lead to the expression of recombinant proteins in the form of inclusion bodies, which cannot meet the needs of obtaining soluble proteins, and the E.coli expression system usually needs to induce the expression of recombinant proteins with IPTG, and IPTG’s The price is more expensive, and the use of IPTG in large-volume production fermentation will also increase the cost

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