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Breeding method of dual-purpose high-resistance starch potatoes

A technology of high resistant starch and resistant starch, applied in the direction of botany equipment and methods, application, plant genetic improvement, etc., can solve the problem of low resistant starch, achieve the effect of reducing the number of samples and reducing the demand for seeds

Active Publication Date: 2022-05-06
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In general, raw potatoes are high in resistant starch (RS2), but cooked potatoes are low in resistant starch (RS3)

Method used

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  • Breeding method of dual-purpose high-resistance starch potatoes
  • Breeding method of dual-purpose high-resistance starch potatoes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Correlation Study of Iodine Staining, RS3, RS2 and Amylose in Potato Tuber and Mature Tuber

[0030] Choose Zhongshu No. 3, Zhongshu No. 5, Zhongshuzao 30, Qingshu No. 5, Qingshu No. 9, Xingjia No. 2, Zheshu 937, Dongnong 303, Zhongzao 45, Zhongshuzao 39, AG370, etc. 10 test-tube small potatoes and mature potato pieces of each of the 11 varieties were used as materials, and the correlation research of iodine staining, RS3, RS2 and amylose was carried out (see Table 1). RS2, amylose were all significantly correlated with mature potato cubes, iodine staining, RS3 and amylose were significantly correlated, while iodine staining, RS3, amylose were not significantly correlated with RS2. Therefore, it is possible to utilize the small potato iodine staining method to carry out early indirect rapid preliminary screening of RS3 of mature potato pieces, and test tube small potato RS2 to carry out the early primary screening of RS2 of mature potato pieces, which not only provides ...

Embodiment 2

[0034] A method for breeding dual-purpose high-resistant starch potatoes, the specific steps are as follows:

[0035] (1) Using Zhongshu No. 3 as the male parent and Zhongshu No. 5 as the female parent, the F 1 Substitute seed 50 grains;

[0036] (2) put F 1 Substitute seedlings, germinate into seedlings on sterile medium, construct potato test tube small potato system, take 781 test tube small potatoes, cut into two pieces, take one piece, and use iodine staining to stain the surface first with Zhongshu No. 3 as a control 183 pieces that were similar to or darker than Zhongshu No. 3 were selected for primary screening, and then the RS2 content was measured, and 32 pieces of small potatoes corresponding to samples with RS2 ≥ Zhongshu No. 5 (43.8%) were selected for miniature potato production. S 1 46;

[0037] (3)S 1 , the strains were planted, the tubers were harvested, the surface was stained with iodine staining method, and 23 strains with the same or deeper staining ...

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Abstract

The invention discloses a method for breeding dual-purpose high-resistance starch potatoes, which comprises the following steps of: hybridizing Zhongshu 5 serving as a male parent and Zhongshu 3 serving as a female parent to obtain F1 seeds; culturing F1 seeds into seedlings, constructing a potato test-tube minituber system, taking test-tube minituber, primarily screening by adopting an iodine dyeing surface staining method, and selecting and remaining the minituber of which the RS2 is greater than or equal to the parent B to obtain S1; the method comprises the following steps: S1, planting a plant line, selecting and remaining a plant line of which the dyeing depth is similar to or deeper than that of Zhongshu No.5, and respectively selecting and remaining a clone S2 of which the RS2 is greater than or equal to the parent B and the RS3 is greater than or equal to the parent A; planting S2, and selecting and remaining clone S3 with RS2 greater than or equal to 40% and RS3 greater than or equal to 5%; s3, planting; S4, selecting and remaining clone with the reducing sugar content less than or equal to that of parents; and constructing a seedling breeding and improved variety cultivation technology of the clone S5, and breeding the dual-purpose high-resistance starch potato. The potatoes obtained through the breeding method can be eaten freshly and can also be puffed, a more perfect industrial chain is expected to be formed, and quality and efficiency improvement of rural areas in the future is promoted.

Description

technical field [0001] The invention relates to the technical field of potato breeding, in particular to a method for breeding dual-purpose high-resistant starch potatoes. Background technique [0002] In 2015, the Ministry of Agriculture and Rural Affairs put forward the strategic concept of potato staple food industrialization, saying that in the future, potatoes will gradually become the fourth staple food crop after potatoes, wheat and corn. However, compared with the diversification of breeding objectives and varieties such as potatoes, wheat, and corn, potato breeding and uses are very single, and there are few special varieties suitable for staple food. In order to fully tap the potential of its staple food consumption and the charm of food innovation, functional potato breeding is the key. [0003] According to the estimates of Healthy China Action, the prevalence of diabetes in my country is 12.8%, with 150 million patients; the prevalence of dyslipidemia is 18.6%,...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04A01H1/12
Inventor 彭张弛吴洪成沈易舒小丽吴殿星
Owner ZHEJIANG UNIV
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