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Cell development map and marker gene of mandible tissue of human three-month-old embryo

A mandible and cell technology, applied in the field of cell development maps and marker genes of human three-month-old embryonic mandibular tissue, to achieve the effect of enhancing reality and accuracy

Inactive Publication Date: 2022-05-06
AFFILIATED HOSPITAL OF NANTONG UNIV
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Problems solved by technology

[0005] However, the developmental map of human embryonic mandibular tissue has not attracted attention and has not been reported. Therefore, the construction of the cell development map of human embryonic mandibular tissue will play an important role in the study of the growth and development of the mandible.

Method used

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  • Cell development map and marker gene of mandible tissue of human three-month-old embryo
  • Cell development map and marker gene of mandible tissue of human three-month-old embryo
  • Cell development map and marker gene of mandible tissue of human three-month-old embryo

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Embodiment Construction

[0025] In order to make the technical problems, technical solutions and advantages to be solved by the present invention clearer, the following will describe in detail in conjunction with specific embodiments.

[0026] This patent performs single-cell sequencing on the human three-month-old embryonic mandibular tissue, and uses UMAP (UniformManifold Approximation and Projection for Dimension Reduction, uniform manifold approximation and projection algorithm) to reduce dimensionality and grouping, and uses the Marker gene (the characteristic expression gene of the cell group) ) to annotate cell populations and construct a cell development map of human embryonic mandibular tissue. The specific steps are:

[0027] 1. Preparation of single cell suspension

[0028] Mandibular tissue was washed three times with Hanks equilibrium solution, cut into small pieces of 1-2mm tissue, and digested in 2ml GEXSCOPETM (Singleron Biotechnologies) tissue dissociation solution at 37°C for 15 min...

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Abstract

The invention provides a cell development map of mandible tissues of human three-month-old embryos. Comprising 19 cell populations including endothelial cells, Schwann cells, proliferating cells, satellite cells, muscle cells, red blood cells, myoblasts, transformed cells 3, transformed cells 1, mesenchymal stem cells, monocytes, osteoclasts, transformed cells 2, neural crest cells, osteoblasts, epithelial cells, chondrocytes, pericytes and tendons. The invention also provides a cell development marker gene of the mandible tissue of the human three-month-old embryo, and the cell development marker gene is characterized by comprising 19 cell populations according to claim 1. The invention further provides application of the cell development marker gene of the human three-month-old embryo mandible tissue in the aspect of judging the development stage of the embryo mandible tissue and the like. The invention provides the cell development map of the mandible tissue of the human three-month-old embryo, and provides new basic data for researching the growth and development of the mandible.

Description

technical field [0001] The invention belongs to the technical field of biological sciences, and relates to a cell development map and a marker gene of human three-month-old embryo mandibular tissue, in particular to a cell development map of human three-month-old embryo mandibular tissue, a human three-month-old embryo mandibular tissue The cell development marker gene of bone tissue and its application in judging the development of embryonic mandibular tissue, and a method for constructing the cell development map of human three-month-old embryonic mandibular tissue. Background technique [0002] Currently, developmental biology research is mainly based on model organisms. Human embryonic development (from zygote to fetal birth) remains a poorly understood "black box" due to practical challenges. Recently, a joint study from multinational research institutions elaborated the roadmap for the construction of the human developmental cell atlas and the reference map of pregnan...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6876C12Q1/6869G16B25/00
CPCC12Q1/6888C12Q1/6876C12Q1/6869G16B25/00C12Q2600/158C12Q2535/122C12Q2537/165C12Q2565/629
Inventor 冯兴梅朱谦齐谭妙盈
Owner AFFILIATED HOSPITAL OF NANTONG UNIV
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