Automatic reagent judgment method based on fluorescence immunoassay and colloidal gold method

A technology of fluorescence immunity and automatic determination, applied in image analysis, image enhancement, instruments, etc., can solve the problems of inability to quantify, non-quantify, and easy fatigue, and achieve the effect of solving manual discrimination errors, meeting practical requirements, and stable and reliable results

Pending Publication Date: 2022-05-17
CHANGSHA XIEDA BIO TECH
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Problems solved by technology

[0003] At present, the determination of fluorescence immunoassay and colloidal gold method mostly adopts manual judgment or semi-automatic instrument judgment. Manual judgment has problems such as low efficiency, dependence on experience, inability to quantify, and easy fatigue. Positive interface is not clear, not quantified, etc.

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  • Automatic reagent judgment method based on fluorescence immunoassay and colloidal gold method
  • Automatic reagent judgment method based on fluorescence immunoassay and colloidal gold method
  • Automatic reagent judgment method based on fluorescence immunoassay and colloidal gold method

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Embodiment 1

[0116] The present invention proposes a fluorescent immunoassay, colloidal gold method reagent automatic determination method:

[0117] S1: call the corresponding configuration parameters according to the reagent type, and collect the image data after the reagent reaction;

[0118] The image of the colloidal gold method reagent is collected by an industrial camera, and the image of the fluorescent reagent is collected by a photosensitive sensor, see figure 2 ; In S1, if the reagent type is judged to be a colloidal gold method reagent, the parameters that need to be configured include: recognition mode RegMode=4, 4 for colloidal gold method, 5 for fluorescence method, number of test lines RegCount=1, yin-yang limit Week_Gray=3.0 That is, the boundary between positive and weak positive, weak positive Gray=20.0 should be set as large as possible, the ratio of positive to weak positive, the boundary between weak positive and negative, pixel value on the left side of ROI BigROI_L=...

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Abstract

The invention discloses a fluorescence immunoassay and colloidal gold method reagent automatic judgment method, which comprises the following steps: firstly, acquiring a reagent image or graph after reaction by adopting a camera or laser excitation, and then carrying out filtering, morphological analysis, base value removal and wave crest detection treatment on the acquired image or graph; the ratio of the quality control line peak area C to the test line peak area T is calculated and compared with a threshold value to determine the strength of a reagent test result, so that automatic judgment of the reagent is realized; the method solves the problems of manual discrimination errors, low efficiency, easy fatigue and the like of reagent reaction results, realizes automatic determination of fluorescence immunoassay and colloidal gold method reagents, rapidly and accurately identifies the results after reagent reaction, is more accurate and stable, can be applied to colloidal gold method series reagents and fluorescence immunoassay series reagents, and has wide application prospects. The method can be used for automatically judging the reaction results of similar reagent products, and can also be used for automatically judging other similar reagents tested by a quality control line and a test line.

Description

technical field [0001] The invention relates to an automatic judgment method for fluorescent immunoassay and colloidal gold reagents, which is especially suitable for automatic judgment of fluorescent immunoassay and colloidal gold reagents. Background technique [0002] Fluorescence immunoassay and colloidal gold reagent automatic determination method is the key technology to realize the automatic and intelligent determination of (C / T) similar reaction types of medical test reagents, which can quickly and accurately calculate the qualitative or quantitative results of the concentration of the detected substance after the reagent reaction. Manual recognition is more accurate and stable. Effectively solve the problems of manual judgment error, low efficiency and easy fatigue of reagent reaction results. [0003] At present, the determination of fluorescence immunoassay and colloidal gold method mostly adopts manual judgment or semi-automatic instrument judgment. Manual judgm...

Claims

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Application Information

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IPC IPC(8): G06T7/00G06T7/13G06T5/00G06T5/30G06T7/136G06T7/194G06T7/62
CPCG06T7/0012G06T7/136G06T7/194G06T5/30G06T7/62G06T7/13G06T2207/10064G06T2207/20061G06T2207/30004G06T5/70
Inventor兰佳王力宇
OwnerCHANGSHA XIEDA BIO TECH