Application and application method of Exiguobacterium P6 keratinase in detergent

A microbacterial and keratinase technology, applied in microorganism-based methods, detergent compounding agents, detergent compositions, etc., can solve the problem of less research on the application of keratinase, and achieve excellent application effect, huge application potential, good The effect of degradation

Active Publication Date: 2022-05-27
VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although there are many reports on the application of common proteases in dete...

Method used

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  • Application and application method of Exiguobacterium P6 keratinase in detergent
  • Application and application method of Exiguobacterium P6 keratinase in detergent
  • Application and application method of Exiguobacterium P6 keratinase in detergent

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Extraction of Exiguobacterium P6 keratinase

[0032] The method for obtaining Exiguobacterium P6 keratinase: The seed solution of Exiguobacterium tumefaciens was inserted into the feather medium according to the inoculum amount of 2%, and after culturing at 25ºC and 180 r / min for 48 h, the cells were centrifuged to obtain keratinase. Medium formula (g / L): Feather 15, NaCl 0.5, K2HPO4 1.0, KH2PO4 0.4, MgCl2 7H2O 0.1, CaCl2 0.06.

[0033] Keratinase enzyme activity determination method: refer to the method of patent ZL201610289190.2.

[0034] Optimum enzyme activity temperature: take 2% keratin as the substrate, dilute the enzyme solution to an appropriate concentration with pH 8.5 Tris-HCl buffer, mix it with the substrate 1:1, and measure the enzyme activity at pH 8.5. The reaction temperature ranges from 30 to 65 °C, and one point is measured every 5 °C, and the highest enzyme activity is taken as 100%;

[0035] Results: The activity of keratinase in catalyzing kerat...

Embodiment 2

[0040] The keratinase of the present invention is applied in removing stains, and the concrete method is as follows:

[0041] Tolerance of keratinase in detergent: keratinase was diluted to 35 U, mixed 1:1 with 2% Bilang detergent, incubated at room temperature for 6 h and 12 h, and then the Folin-phenol method was used. Measure residual enzyme activity. The enzyme activity of keratinase not mixed with detergent at 0 h was taken as 100%.

[0042] The washing effect of keratinase on stains: The concentration of Bilang detergent is 1%, adding 35 U of enzymes, and cutting the stained cotton cloth into a suitable size. Washing conditions: 37°C, 120 rpm for 1 h. Compare the washing effect of distilled water and Bilang detergent without adding enzyme liquid.

[0043] The enzyme activity of keratinase remained stable when incubated in 1% Bilang detergent for 12 h (attached). figure 2 ), compared with Bilang detergent with a mass fraction of 1% without keratinase, the detergent w...

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Abstract

The invention belongs to the field of washing enzymes of industrial enzyme preparations, and particularly relates to an application and an application method of Exiguobacterium P6 keratinase in a detergent, the strain is preserved in China General Microbiological Culture Collection Center on March 01, 2022, the preservation number of the strain is CGMCC No.24453, and the preservation number of the strain is CGMCC No.24453. The invention further discloses a preparation method of the Exiguobacterium P6 keratinase. The preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The keratinase provided by the invention is obtained by inoculating a seed solution of Exiguobacterium P6 into a feather culture medium for culture, belongs to medium-temperature alkaline protease, has good stress resistance, can tolerate ionic/nonionic surfactants, oxidants and bleaching agents, can maintain stable enzyme activity for a long time in commercially available detergents, and can be used for preparing the feather feather keratinase. The cleaning agent has excellent decontamination, dirt removal and stain removal effects on common stains.

Description

technical field [0001] The invention belongs to the field of washing enzymes for industrial enzyme preparations, and in particular relates to a microbacillus strain Exiguobacterium sp. Application and application method of P6 keratinase in detergent. Background technique [0002] Commonly used enzyme additives in the detergent industry include protease, amylase and lipase, each suitable for removing different types of soils. Regardless of the sophistication of current detergents, there is still an urgent need to develop new detergents including new enzymes / enzyme blends to meet industry needs. [0003] Compared with ordinary protease, keratinase can more easily wash off the keratinized waste on the surface of clothes, and can be used as an additive for detergents. This application requires protease with good alkali resistance, high activity in a wide temperature range, good cleaning effect on stains, and resistance to ionic / nonionic surface activity in detergents agents,...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/52C11D3/386C12R1/01
CPCC12N9/52C11D3/38609C11D3/38618
Inventor 边斐岳寿松张燕朱友峰于金慧陈高马德源
Owner VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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