Two-step SERS (Surface Enhanced Raman Scattering) detection method for judging novel coronavirus infectivity in environment
A technology for coronavirus, detection methods, applied in the directions of microorganism-based methods, biochemical equipment and methods, determination/inspection of microorganisms, etc.
Pending Publication Date: 2022-06-10
SHANGHAI INST OF CERAMIC CHEM & TECH CHINESE ACAD OF SCI
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As a result, the non-infectious and cracked new coronavirus is misdiagnosed as an infectious virus source, causing unnecessary social panic and cost consumption
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Abstract
The invention relates to a two-step SERS (Surface Enhanced Raman Scattering) detection method for judging novel coronavirus infectivity in an environment, which comprises the following steps: selecting an SERS enhanced substrate, carrying out SERS detection on SARS-CoV-2 S protein and RNA (Ribonucleic Acid), and constructing a judgment standard according to the difference of SERS signals of the SARS-CoV-2 S protein and RNA; and performing classification treatment on Raman spectrums obtained by performing two-time SERS detection on a new coronavirus sample existing in the environment in combination with a machine learning method so as to judge the infectivity of the new coronavirus existing in the environment.
Description
[0001] The present invention relates to a two-step SERS detection method for judging the infectivity of novel coronaviruses in the environment, in particular to an identification standard constructed based on the SERS signal difference between SARS-CoV-2S protein and RNA, and a two-step SERS method is designed The detection method to judge the infectivity of SARS-CoV-2 in various virus-infected environments such as cold chains and express delivery belongs to the field of laser Raman spectroscopy detection technology and biological virus sensing. Background technique [0002] At present, the new crown epidemic is facing the dilemma of globalization and time continuity. Moreover, the detection of SARS-CoV-2 virions on cold chain or express items has aroused high social attention to a new round of virus transmission. A series of detection methods have been developed for SARS-CoV-2 at home and abroad, among which the real-time fluorescent quantitative PCR technology is the most wid...
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IPC IPC(8): G01N21/65G06K9/62G06N20/00C12Q1/70C12R1/93
CPCG01N21/658C12Q1/70G06N20/00G01N2021/655G06F18/2135G06F18/2411G06F18/214C12Q2565/632
Inventor 杨勇彭宇思黄政仁
Owner SHANGHAI INST OF CERAMIC CHEM & TECH CHINESE ACAD OF SCI
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