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Mumps virus attacking animal model and establishment method thereof

A mumps virus and animal model technology, which is applied to the mumps virus challenge animal model and the field of its establishment, can solve the problems of inability to form and the mumps virus infection efficiency is not high, and achieves the effect of simple operation

Active Publication Date: 2022-07-29
BEIJING CELL FUSION BIOTECHNOLOGY CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the infection efficiency of mumps virus is not high in various non-human animal models, and it cannot cause symptoms similar to human infection (for example, significant replication in various organs). Method for constructing mumps virus challenge animal model for effective infection of mumps virus, so as to be used for evaluating the effectiveness of mumps virus vaccine

Method used

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  • Mumps virus attacking animal model and establishment method thereof
  • Mumps virus attacking animal model and establishment method thereof
  • Mumps virus attacking animal model and establishment method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Example 1: Effect of mouse type on morbidity after mumps virus challenge

[0071] The experimental animals were IFNα / βR- / - gene deletion mice (4-6 weeks old, from Huazhong Agricultural University) and 129 mice (4-6 weeks old, purchased from Beijing Weitong Lihua Company). 6 groups, 4 mice in each group, 6 experimental groups were IFNα / βR- / -gene-deficient mice challenged by tail vein, intramuscular and intranasal injection of F genotype mumps virus strain QS-F virus solution 129 mice were challenged by tail vein, intramuscular and intranasal injection of the virus solution of the F genotype mumps virus strain QS-F, and the challenge dose was 1 × 10 6 CCID 50 / Only. 14D after challenge, the blood, lymph nodes, spleen, liver, heart and kidney of mice with IFNα / βR- / - gene deletion in experimental group and 129 mice were collected for quantitative PCR, and IFNα / βR- / - The viral load of mumps virus in blood, lymph nodes, spleen, liver, heart and kidney of gene deletion mice...

Embodiment 2

[0082] Example 2: Effect of challenge dose on morbidity after mumps virus challenge

[0083] The experimental animals were IFNα / βR- / - gene-deficient mice (4-6 weeks old, from Huazhong Agricultural University). The experimental group consisted of 4 groups, each with 4 mice. The 4 groups were all injected with F genotype through tail vein injection. IFNα / βR- / -gene-deficient mice were challenged with the viral solution of mumps virus strain QS-F, and the challenge dose was 1 × 10 3 CCID 50 / only, 1 × 10 4 CCID 50 / only, 1 × 10 5 CCID 50 / only 1×10 6 CCID 50 / Only. After challenge, 3D, 7D, 14D and 28D were collected from the blood of mice with IFNα / βR- / - gene deletion in the experimental group for real-time quantitative PCR (see Example 1 for real-time quantitative PCR), and the experimental group IFNα / βR- / - The viral load of mumps virus in the blood of gene deletion mice, the detection results are shown in Table 3.

[0084] As can be seen from Table 3, 3D after challe...

Embodiment 3

[0087] Example 3: Mumps virus challenge animal model for evaluation of strain virulence

[0088] The evaluation objects are A genotype mumps virus strain JL, F genotype mumps virus strain QS-F, F genotype mumps virus strain QS-F-SH2 and G genotype mumps virus strain MuV (G genotype mumps virus strain QS-F-SH2). genotype), the experimental animals were IFNα / βR- / - gene-deficient mice (4-6 weeks old, from Huazhong Agricultural University). The experimental group consisted of 4 groups with 4 mice in each group. A genotype mumps virus strain JL, F genotype mumps virus strain QS-F, F genotype mumps virus strain QS-F-SH2 and G genotype mumps virus strain MuV (G genotype) IFNα / βR- / -gene-deficient mice challenged with virus fluid, the challenge dose is 1×10 3 CCID 50 / Only. 14D after the challenge, the blood of mice with IFNα / βR- / - gene deletion in the experimental group was collected for quantitative PCR (see Example 1 for fluorescence quantitative PCR), and the mice with IFNα / βR- / ...

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Abstract

The invention relates to a mumps virus attacking animal model and an establishment method thereof, and belongs to the technical field of biology. The invention provides a mumps virus counteracting animal model. The mumps virus counteracting animal model is an interferon receptor deletion animal which is intravenously injected with mumps virus. Research finds that compared with common mice, the mumps virus load in blood of the interferon receptor-deleted mice is remarkably improved after the mumps virus is attacked by the interferon receptor-deleted mice, and compared with intramuscular injection and intranasal injection, the mumps virus load in blood of the interferon receptor-deleted mice is remarkably improved after the mumps virus is injected into the interferon receptor-deleted mice through caudal veins, so that the mumps virus load in blood of the interferon receptor-deleted mice is remarkably improved. Intravenous injection of the mumps virus to the interferon receptor-deficient animal can break through species limitation, effective infection of the mumps virus in the animal body is realized, and symptoms similar to human infection (for example, remarkable replication in various visceral organs) are formed, so that the mumps virus challenge animal model can be used as the mumps virus challenge animal model.

Description

technical field [0001] The invention relates to a mumps virus challenge animal model and a method for establishing the same, belonging to the field of biotechnology. Background technique [0002] Mumps is an acute infectious disease caused by mumps virus (MuV), which is a common respiratory infectious disease in children and adolescents. Its main clinical symptoms are swelling and pain in the parotid gland, and sometimes it can also cause acute or systemic inflammation such as viral encephalitis, orchitis, pancreatitis or oophoritis. [0003] MuV is mainly transmitted through saliva, saliva-contaminated items, and air droplets. Mumps is one of the world's most prevalent infectious diseases, with a wide geographical distribution. The prevalence and outbreak of mumps have no obvious seasonal and regional characteristics, and are not affected by factors such as climate, but the number of cases in winter and spring is more than that in summer. [0004] At this stage, the main...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027A61K49/00
CPCA01K67/027A61K49/0008A01K2207/20A01K2227/105A01K2227/107A01K2227/10A01K2267/03
Inventor 安祺田大勇刘飞张亚静
Owner BEIJING CELL FUSION BIOTECHNOLOGY CO LTD
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