Separation device for cells and metabolites thereof

Abstract

The invention discloses a cell and metabolite separation device which comprises a device body, the device body comprises a reactor, a container, a peristaltic pump, a hollow fiber membrane column, a connecting pipe, a photoelectric sensor, a glass tube, a sterile filtering device, a control valve, an air compressor and a vacuum pump, and the reactor is connected with the hollow fiber membrane column through a pipeline; the side face of the hollow fiber membrane column is connected with one end of the peristaltic pump through a pipeline, the other end of the peristaltic pump is connected with the container through a pipeline, the hollow fiber membrane column and the glass pipe are symmetrically installed at the left end and the right end of the top of the connecting pipe, and the top of the glass pipe is connected with the sterile filtering device through a pipeline. And the bottom of the sterile filtering device is connected with the control valve through a pipeline. According to the device, an existing diaphragm pump and an existing diaphragm for filtering the cells are omitted, so that liquid flows more softly when the cells run, the cells are prevented from being damaged when being filtered, the shearing force of the system is reduced, the risk of damage to the diaphragm is reduced, and the investment cost of the system is reduced.

Description

technical field [0001] The invention relates to the technical field of cell separation, in particular to a cell and metabolite separation device. Background technique [0002] Cell culture refers to a method that simulates the in vivo environment (sterility, suitable temperature, pH and certain nutrient conditions, etc.) in vitro to make it survive, grow, reproduce and maintain its main structure and function. Cell culture is also called Cell cloning technology, the formal term in biology is cell culture technology. Whether it is for the entire bioengineering technology or one of the biological cloning technologies, cell culture is an essential process, and cell culture itself is a cell. Large-scale cloning, cell culture technology can transform a cell into a simple single cell or minimally differentiated multi-cell after a large number of cultures, which is an essential part of cloning technology, and cell culture itself is the cloning of cells, cell culture technology It ...

AI Technical Summary

Problems solved by technology

[0004] 1. When the existing cell filtration device is in use, the power of the liquid flow is provided by the diaphragm pump and the diaphragm, and the cells are vulnerable to damage during filtration.

[0005] 2. The existing cell separation device relies on the diaphragm in the diaphragm pump to ensure the aseptic state of the system, and the diaphragm is easily damaged, which can easily lead to cell culture failure

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