Multi-effect personal care compositions and methods thereof
A personal care and composition technology, applied in multi-effect personal care composition and its field, can solve the problem that the search for new beneficial agents is still in progress
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example 1
[0062] An in vitro study was performed on a keratinocyte skin model to determine the efficacy of different concentrations of hemp seed oil (HSO) and / or flax seed oil (FSO) on the production of antimicrobial peptides (AMPs). AMP production was determined by measuring the presence of an AMP biomarker, LL-37. It is understood that AMPs, also known as host defense peptides, are part of the innate immune response. AMP has demonstrated efficacy as a therapeutic agent and has further demonstrated its ability to treat, reduce or eliminate gram-negative and gram-positive bacteria, including conventional antibiotics, mycobacteria, enveloped viruses, fungi, and transformed Those bacteria to which cells or cancer cells are resistant. From the foregoing, it is understood that AMPs provide protection of the epithelial surface or skin against environmental microorganisms.
[0063] To prepare different concentrations or dilutions of HSO and / or FSO, stock solutions of HSO and FSO were prepar...
example 2
[0074] An in vitro study was performed on a keratinocyte skin model to observe the production of interleukin 1α (IL-1α) in the presence of various concentrations of HSO and / or FSO. It will be appreciated that IL-1α, also known as hematopoietin 1, is a cytokine of the interleukin 1 family encoded by the IL1α gene. It is further understood that interleukin 1 is responsible for the production of inflammation and irritation and is widely regarded as a biomarker for assessing skin irritation.
[0075] To assess IL-1α production, keratinocyte skin models were grown to at least 60% confluence and placed in corresponding wells of well plates. Stock solutions of FSO and HSO were prepared analogously to Example 1. Specifically, 100% FSO was combined with 100% dimethyl sulfoxide (DMSO) in a ratio of about 1:99, thereby preparing a 1% solution of FSO in DMSO. Similarly, a 1% solution of HSO in DMSO was prepared by combining HSO with 100% DMSO in a ratio of about 1 :99. A vehicle stock ...
example 3
[0089] A similar in vitro study to Example 2 was performed on a keratinocyte skin model to observe the production of interleukin 1α (IL-1α) in the presence of various concentrations of cannabis HSO and / or FSO. The stock solution prepared in Example 2 was used in the study. The stock solutions were used to prepare eight samples (15)-(22) with varying amounts of FSO and / or HSO. The amounts or concentrations of each of the samples (15)-(22) are summarized in Table 6. A brief description of each of the samples (15)-(22) is summarized in Table 7.
[0090] Table 6
[0091] Composition of samples (15)-(22)
[0092]
[0093] Table 7
[0094] Brief description of samples (15)-(22)
[0095] sample brief introduction 15 negative control 16 0.0005% FSO 17 0.001% HSO 18 0.0005% HSO 19 0.00025% HSO 20 0.0005%FSO+0.001%HSO 21 0.0005%FSO+0.0005%HSO 22 0.0005%FSO+0.00025%HSO
[0096] Each of the samples (15)-(22) was used...
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