Myelodysplastic syndrome drug-resistant cell model as well as construction method and application thereof

A drug-resistant cell and construction method technology, applied in the field of myelodysplastic syndrome drug-resistant cell model and its construction, can solve the problem of unclear drug resistance mechanism of demethylated drugs, and achieve the effect of stable drug resistance

Pending Publication Date: 2022-08-09
LUOYANG CENT HOSPITAL
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, the mechanism of resistance to hypomethylating drugs is still unclear

Method used

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  • Myelodysplastic syndrome drug-resistant cell model as well as construction method and application thereof
  • Myelodysplastic syndrome drug-resistant cell model as well as construction method and application thereof
  • Myelodysplastic syndrome drug-resistant cell model as well as construction method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] This embodiment provides a myelodysplastic syndrome drug-resistant cell model and its construction method and use.

[0028] 1. Materials and methods

[0029] 1. Cell lines and main reagents

[0030] Human MDS cell lines (SKM-1, MOIM-13) were purchased from the National Experimental Cell Resource Sharing Service Platform, passaged and preserved in the Hematology Laboratory of Luoyang Central Hospital;

[0031] Fetal bovine serum (Hycolne, USA), RPMI-1640 medium (Hycolne, USA), Penicillin-streptomycin (Invitrogen, USA), Glutamine (Invitrogen, USA), Cell Counting Kit-8 (Beijing Soleibo) Technology Co., Ltd.), Annexin V-FITC Apoptosis Detection Kit (Beijing Soleibao Technology Co., Ltd.), azacitidine (TaKaRa Company).

[0032] 2. Experimental method

[0033] (1) Cell culture

[0034] SKM-1 and MOIM-13 cells used in this experiment were cultured with RPMI-1640+15% fetal bovine serum medium, and 1% double antibody (penic-streptomycin) and 1% glutamine were added at the sa...

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Abstract

The invention belongs to the technical field of cell drug resistance, and relates to a myelodysplastic syndrome drug-resistant cell model and a construction method and application thereof, and the cell model is established by adopting a method of gradually increasing drug concentration and intermittently inducing and is formed by azacitidine induction. According to the present invention, the MDS AZA-resistant cell strains SKM-1-AZA and MOIM-13-AZA are successfully induced, the growth and the drug resistance of the MDS AZA-resistant cell strains are stable, and the experimental basis is provided for the further discussion of the drug resistance mechanism.

Description

technical field [0001] The invention belongs to the technical field of cell drug resistance, and relates to a myelodysplastic syndrome drug resistance cell model and a construction method and application thereof. Background technique [0002] Myelodysplastic Syndrome (MDS) is a group of clonal hematopoietic stem cell diseases characterized by one or more lineages of abnormal hematopoiesis and ineffective hematopoiesis. The probability of transformation into acute myeloid leukemia is as high as 30%. Studies have found that the pathogenesis of MDS is closely related to epigenetic abnormalities, and DNA methylation abnormalities are the most common epigenetic changes in MDS. In recent years, epigenetic therapy represented by the demethylating drug azacitidine (AZA) has shown good efficacy in MDS. However, the phenomenon of demethylation drug resistance is inevitable. Most patients develop drug resistance within 2 years of treatment response. The median overall survival (OS) of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0789
CPCC12N5/0647C12N2501/999
Inventor 王慧睿毛慧云郭淑利刘思哲安万花
Owner LUOYANG CENT HOSPITAL
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