Biological agent for reducing specific nitrosamine content which tobacco has, preparation method and use

A technology of biological agents and nitrosamines, which is applied in the biological field and can solve problems that have not been widely applied

Inactive Publication Date: 2006-10-11
YUNNAN ACAD OF TOBACCO AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the limitations of these methods, they have not been widely applied at present.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Inoculate Pseudomonas aeruginosa (Pseudomonas aeruginosa) KenLXP30 bacterial strain on the agar slant of the test tube, the medium formula is: casein 1.2%, soybean peptone 0.8%, sodium chloride 0.5%, agar 1.7%, pH7.5. 30 ℃ Incubate at constant temperature for 1 day to obtain test tube seeds.

[0047] The test tube is inoculated into a 500ml Erlenmeyer flask (200ml per bottle) liquid medium, and the medium formula is: casein 1.5%, soybean peptone 0.5%, sodium chloride 0.5%, dipotassium hydrogen phosphate 0.025%, glucose 0.3%, pH7.5. Cultivate the seeded Erlenmeyer flask at 30°C on a shaker (180rpm) for 2 days, until the concentration of bacteria reaches the number of viable bacteria per ml ≥ 10 8 , the biological agent with the function of reducing TSNA can be obtained.

Embodiment 2

[0049] Basically the same as Example 1, the difference lies in the formula of the liquid medium, which formula is: 1% casein, 0.8% soybean peptone, 0.2% sodium chloride, 0.2% dipotassium hydrogen phosphate, 0.6% glucose, pH7.

Embodiment 3

[0051] Basically the same as Example 1, the difference lies in the formula of the liquid medium, which is: 2% casein, 0.3% soybean peptone, 0.7% sodium chloride, 0.4% dipotassium hydrogen phosphate, 0.2% glucose, pH 7.2.

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PUM

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Abstract

The invention relates to a biological preparation for reducing the content of tobacco-specific nitrosamines and a preparation method thereof, belonging to the field of biological technology. The production strain Pseudomonas aeruginosa KenLXP30 of the present invention has been preserved in the General Microorganism Center of China Microorganism Culture Collection Management Committee on July 28, 2003, with the preservation number CCTCC NO.0985. The invention screens out a bacterial strain KenLXP30 that can significantly reduce the content of tobacco-specific nitrosamines from a large number of tobacco endophytic bacteria, and prepares tobacco pest-reducing biological preparations through liquid fermentation. The preparation can significantly reduce the content of specific nitrosamines in Burley tobacco, and has good application value in the tobacco industry.

Description

Technical field: [0001] The invention relates to a biological preparation for reducing the content of tobacco-specific nitrosamines, a preparation method and an application thereof, and belongs to the field of biological technology. Background technique: [0002] Tobacco-specific nitrosamines (TSNA) are a group of carcinogens found only in tobacco and its products, mainly including N-nitrosonornicotine (NNN), 4-(N-formazine 4 ingredients. Among them, NNN and NNK have significant carcinogenic effects, while NAB and NAT have no obvious carcinogenic effects. Burley tobacco had the highest content of TSNA in different tobacco types, and the content of oriental tobacco and flue-cured tobacco was lower. Studies have shown that the main precursors of TSNA are alkaloids, nitrate, and nitrite. There is no TSNA in the green tobacco leaves before harvest, and they are produced during the modulation and fermentation process after harvest. Over the past 20 years, TSNA has become one ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A24B15/20A61K39/104
Inventor 祝明亮李天飞汪安云王东丹
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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