Making and planting method for honeysuckle microbe bacteria
The technology of a microbial strain and a cultivation method is applied in the field of honeysuckle microbial strain production and cultivation, and can solve the problems of harsh cause conditions, artificial domestication, reproduction and cultivation, and difficulty in meeting the needs of social life.
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Embodiment 1
[0012] Carefully select natural wild fruit bodies that are complete, thick and firm, with little moisture, and free of diseases and insect pests. Use a scalpel to cut out the vigorous and vigorous bacterial clumps that are slightly inside the edge of the fruit body tissue, and pick a few of the soy bean-sized bacterial pith. Then use tweezers to move the hyphae into the test tube culture medium, place the test tube obliquely downwards, use black or gray hard paper to make a bag suitable for the thickness of the test tube, and place it at 22~26℃. Cultured under the conditions, the hyphae can grow on the tissues after 5-7 days. After removing the hyphae infected by the miscellaneous bacteria, continue to cultivate until the hyphae are basically overgrown on the slope of the medium, and the rice grain size can be picked (about 1% of the slope). / 30) Transplant the inoculum block to another new culture medium, place it at 22~26℃, and then grow the entire test tube slope in about two w...
Embodiment 2
[0014] Select natural wild old honeysuckle microbial fruiting bodies that grow well, no other wood-rot fungi are mixed, and the substrate vines are surrounded by the stalk base of the fruiting bodies. Use a pruning knife to cut a piece of mycelium-dense mycelium, and remove the dirty part of the outer layer , Then move into the sterilized inoculation box, burn with an alcohol lamp for 5-10 seconds to burn off the bacteria on the surface of the fungus, and then make the fungus into a needle shape, and use tweezers to move the middle part into a test tube with culture medium Put the cotton plug in the middle and place it at 22~26℃ for 3~4 days. At this time, mycelium will grow on the fungus. After cultivating for 7-8 days, take the rice-sized healthy hyphae and transfer to another new one. The whole test tube slope can be grown by placing it on the culture medium at 22~26℃ for about two weeks, and then dividing it into 20 parts and placing them in plastic bags containing culture mat...
Embodiment 3
[0016] Collect wild honeysuckle microbial cells with large cells, thick flesh, dense outer luster, no pests and diseases, mature and beginning to emit spores, and place them in an inoculation box, place them on an enamel vessel, and use sterilized gauze to remove Cover it, then move it to the culture room and cultivate at 18-22℃. After 1 to 2 days, the spores will be scattered on the enamelware underneath. Then move the spores together with the container to the inoculation box, and use the pulling needle to get it. The spores are connected to the test tube culture medium and gently stroked to make the spores evenly distributed on the culture medium, and cultivated at 22~26℃ until the spores germinate and grow into hyphae. Eliminate unhealthy hyphae. Then put it at 24℃ to continue the culture until the test tube is full, and then expand the culture.
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