Making and planting method for honeysuckle microbe bacteria
The technology of a microbial strain and a cultivation method is applied in the field of honeysuckle microbial strain production and cultivation, and can solve the problems of difficult to find and collect, harsh conditions for formation, artificial domestication, reproduction and cultivation, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0014] Carefully select natural wild fruiting bodies that are complete, thick and firm, low in water, and free from diseases and insect pests. Use a scalpel to cut off the vigorously growing and strong newborn bacteria blocks at the edge of the fruiting body, and pick a few bacteria from the soybean grain-sized marrow. Then use tweezers to move it into the culture medium of the test tube, put the inclined side of the test tube down, use black or gray hard paper to make a bag suitable for the thickness of the test tube, cover it, and then put it in the condition of about 22-26°C After 5-7 days, hyphae can grow on the tissue. After removing the mycelia infected by miscellaneous bacteria, continue to cultivate until the mycelium is basically covered with the slant of the medium, and then the rice grain size (about 1 / 30 of the slant) can be picked. ) transplanted the inoculated block to another new medium, cultured at 22-26°C, and the entire slope of the test tube can be covered in...
Embodiment 2
[0016] Select the natural wild old honeysuckle microbial fruiting body that grows well, without other wood-rot fungi mixed with woody vines surrounded by the stalk base of the fruiting body, use a pruning knife to cut a piece of fungus wood with dense mycelium, and remove the dirty part of the outer layer , then move it into a sterilized inoculation box, burn it with an alcohol lamp for 5-10 seconds, burn off the bacteria on the surface of the fungus wood, then make the fungus wood into a wooden needle shape, and use tweezers to move the middle part into a test tube with medium Then plug the cotton plug and culture it at 22-26°C for 3-4 days. At this time, mycelium will grow on the fungus wood. After continuing to cultivate for 7-8 days, take a healthy mycelium the size of a grain of rice and move it into another new one. Then put it on the medium of 22~26℃ and cultivate it for about two weeks to cover the whole slope of the test tube, and then divide it into 20 parts and put t...
Embodiment 3
[0018] Collect the fruiting body of wild honeysuckle microorganisms that are large, fleshy, glossy on the outside and dense on the inside, free from diseases and insect pests, mature and begin to emit spores, put them in the inoculation box, put them on the enamel container, and then use sterilized gauze Cover it, then move it to the culture room and cultivate it at 18-22°C. After 1-2 days, the spores will be scattered on the enamel vessel below, and then move the spores together with the vessel to the inoculation box, and use the inoculation needle to pick up the spores , connected to the test tube culture medium and gently paddling to distribute the spores evenly on the culture medium, and culture at 22-26°C until the spores germinate and grow into hyphae. Remove unhealthy hyphae. Then put it at 24°C and continue to cultivate until the test tube is full, and then expand the culture.
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More