Vaccine-induced hepatitis B viral strain and uses thereof

A hepatitis B virus, virus genome technology, applied in the direction of viruses, antiviral agents, sensory diseases, etc., can solve problems such as transmission of viruses

Inactive Publication Date: 2001-07-18
GOVERNMENT OF THE REPUBLIC OF SINGAPORE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The presence of HBsAg is used as the primary detection marker for HBV carriers who may be at risk of transmitting the virus

Method used

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  • Vaccine-induced hepatitis B viral strain and uses thereof
  • Vaccine-induced hepatitis B viral strain and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0109] The nucleotide sequence and induced amino acid sequence of the hepatitis B virus with a vaccine-induced mutation (glycine to arginine) at amino acid residue 145 of the major surface antigen were determined as follows: 1. Isolation of viral DNA

[0110] Viral DNA was isolated from a serum sample (20 / 815c) obtained from an 11-year-old Chinese child born to a mother carrying wild-type surface antigen. Despite a negative test result at birth, the child received a combination of HBIG and a plasma-derived vaccine. He was tested positive for hepatitis B virus surface antigen one year later, and sequence analysis of the "a" determinant revealed a vaccine-induced mutation at amino acid residue 145 of the major antigen.

[0111] The separation method used was:

[0112] Add 400ul of dissipating buffer (Tris-Cl 10mM, pH7.4, EDTA 1mM, and sodium lauryl sulfate 2%) and 25ul of proteinase K (20mg / ml) to 200ul serum samples, incubate at 65°C for 3 hours . Viral DNA was then extract...

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Abstract

This invention provides an isolated strain of Hepatitis B virus designated Human Hepatitis B Virus Surface Antigen-'S'-145 Singapore Strain (Glycine to Arginine) which constituent viral genome is deposited under Accession Nos. P97121504, P97121505 and P97121506 with the European Collection of Cell Culture on 15th Dec. 1997. This invention also provides an isolated nucleic acid encoding a polypeptide which is a mutant major surface antigen of a strain of hepatitis B virus, such polypeptide having an amino acid sequence which differs from the amino acid sequence of a major surface antigen of a wild type hepatitis B virus in that the amino acid at position number 145 of such polypeptide is an arginine rather than a glycine, and the purified peptide This invention further provides an isolated nucleic acid which encodes a peptide, wherein the peptide is encoded by a nucleic acid molecule comprising nucleotides 527 through 595 of SEQ. I.D. No. 1, and the purified peptide. This invention also provides various methods using the disclosed isolated nucleic acids and polypeptides.

Description

[0001] Throughout the application, various references are given in parentheses. The disclosures of these publications in their entirety are hereby incorporated by reference herein to more fully describe the state of the art to which this invention pertains. Background of the invention [0002] The present invention relates to the hepatitis B virus genome with a vaccine-induced mutation (changing glycine to arginine) at the 145th amino acid residue in the main surface antigen, its nucleotide sequence, and four main protein sequences induced , antigens, antibodies, detection systems, development of effective vaccines and antiviral reagents. [0003] First discovered in 1963, hepatitis B virus is a parenterally transmitted human virus. Although these viruses are not significantly cytotoxic and do not cause massive cell death, they are the cause of a major infectious disease affecting both adults and children worldwide. The presence of HBsAg is used as the primary detection mark...

Claims

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Application Information

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IPC IPC(8): C12N15/09A61K38/00A61K38/17A61K38/22A61K39/00A61K39/29A61K45/00A61K48/00A61P1/16A61P9/00A61P11/06A61P13/12A61P17/00A61P17/02A61P19/02A61P27/02A61P29/00A61P31/00A61P31/14A61P31/20A61P35/00A61P43/00C07K14/02C07K14/71C07K16/00C07K16/08C07K19/00C12N1/19C12N1/21C12N5/10C12N7/00C12N7/01C12N15/12C12N15/51C12N15/62C12P21/02C12P21/08C12Q1/68C12Q1/70G01N33/53G01N33/566G01N33/576
CPCC07K2319/00A61K39/29C07K14/71C12N2730/10122C07K14/005C12Q1/706C12N7/00G01N33/5764A61K39/00C07K2319/30C12N2730/10134A61K39/12A61P1/16A61P11/06A61P13/12A61P17/00A61P17/02A61P19/02A61P27/02A61P29/00A61P31/00A61P31/14A61P31/20A61P35/00A61P43/00A61P9/00
Inventor 温崇仁林玉娇梁爱玲赵奕陈维宁
Owner GOVERNMENT OF THE REPUBLIC OF SINGAPORE
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