Engineered scaffold of amniotic membrane tissue and process for removing cells from aniotic membrane

A technology of tissue engineering scaffolds and amniotic epithelial cells, which can be applied to artificial cell constructs, tissue culture, animal cells, etc., can solve the problems of inability to transplant into the body, inability to use tissue engineering scaffolds, and inability to plant other cells, etc., to achieve a good society The effect of benefit, low cost and simple method

Inactive Publication Date: 2002-09-18
GENERAL HOSPITAL OF TIANJIN MEDICAL UNIV
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Problems solved by technology

The amniotic membrane has no blood supply, the basement membrane layer and the dense layer have no cellular components, and has good ductility and high mechanical strength. It is a natural tissue engineering scaffold, but because one side of the amniotic membrane is covered by columnar epithelial cells,

Method used

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[0015] How to remove the epithelial cell layer and fibroblasts of the amniotic membrane is the key to the use of the amniotic membrane as a scaffold material for tissue engineering, and is also a key problem solved by the present invention.

[0016] For example, the amniotic membrane can be decellularized by the following method to obtain the amniotic membrane with the epithelial cells of the epithelial cell layer removed:

[0017] (1) Use 0.2% trypsin solution to soak the amniotic membrane at 37°C for 25 minutes, or take any combination within the range of 0.2-5% trypsin solution and soak for 20-30 minutes;

[0018] (2) Fix the amniotic membrane, stir and rinse during or after soaking to wash away the epithelial cells of the amniotic membrane epithelial cell layer and the fibroblasts of the fibroblast layer. If the washing is not complete, use a cell scraper to scrape off the remaining Surface cells.

[0019] Use the following methods to decellularize the amniotic membrane to obt...

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Abstract

An engineered scaffold of tissue is prepared from the amniotic membrane whose epicytes and fibrous matericytes has been removed. A process for removing said cells from amniotic membrane includes immersing the amniotic membrane in 0.2-5% trypsase solution at 37 deg.C for 20-25 min and stirring while flushing. Its advantages are no immunoreaction and simple process.

Description

technical field [0001] The invention relates to the field of tissue engineering for manufacturing human organs and tissues, in particular to an amniotic membrane tissue engineering scaffold used as a tissue engineering scaffold and an amniotic membrane decellularization method. Background technique [0002] Tissue engineering research is the science of manufacturing human organs and tissues. Due to its broad application prospects and increasing industrial benefits year by year, governments and industries of various countries have invested heavily in research and industrial construction. The world's annual research funding is more than billions of dollars. my country's 863,973, climbing plan has also invested several hundred million yuan in research. Tissue engineering research includes two main aspects: one is the scaffold used to build organs and tissue shapes; the other is the cells that can grow and play physiological functions on the scaffold. For tissue engineering sc...

Claims

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Application Information

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IPC IPC(8): C12N5/00C12N5/071
Inventor 雪原
Owner GENERAL HOSPITAL OF TIANJIN MEDICAL UNIV
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