Broad spectrum bacillus thuringiensis and the method for preparing pesticide therewith

A technology of Bacillus thuringiensis and Bacillus aureus, which is applied in the fields of botanical equipment and methods, biocides, bacteria, etc., can solve the problems of the isolation and application of Bacillus thuringiensis, which are rarely reported, and achieve good production technology, expand the scope of use, increase Yield effect

Inactive Publication Date: 2003-01-22
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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AI Technical Summary

Problems solved by technology

However, the isolation and application of natural broad-spectrum and high-efficiency Bacillus thuringiensis are still rarely reported.

Method used

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Examples

Experimental program
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specific Embodiment approach

[0042] (1) 2 tons of fermentation medium are put into 3 tons of fermenter; Corn steep liquor 2.0, MgSO 4 7H 2 O 0.04, KH 2 PO 4 0.2,K 2 HPO 4 0.2, CaCO 4 0.1, α-amylase 0.01, the rest is water;

[0043] (2) adjust the pH value of the culture medium to 7.8 with NaOH;

[0044] (3) Steam sterilization at 120°C for 30 minutes;

[0045] (4) putting 25 grams of bacterial classification into warm water at 60° C. for 30 minutes to make a spore suspension;

[0046](5) When the temperature of the culture medium drops to 37° C., the spore suspension is inserted into the fermenter;

[0047] (6) ferment for 10 hours under the conditions of 32°C, 400 rpm, and ventilation rate of 2 cubic meters per minute;

[0048] (7) ferment for 12 hours under the conditions of 29°C, 500 rpm, and ventilation volume of 2.5 cubic meters per minute;

[0049] (8) ferment for 15 hours at 32°C, 500 rev / min, and 2.5 cubic meters per minute of ventilation;

[0050] (9) Terminate the fermentation whe...

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Abstract

The present invention discloses a strain of broad spectrum bacillus thuringiensis, Hcy08-02, preserved in the Chinese typical culture article preserving center in Wuhan University with the CCTCC No.M202025. It has excellent fermentation property, contains several pesticidal protein encoding genes and has effective killing function to cotton bollworm, cabbage moth, small mottid willow moth, cabbage worm and other pests. The present invention also discloses the method of utilizing the strain in producing pesticide to kill several kinds of lepidoptera pests. The strain and pesticide have no harmto human body, farm animal and plant, no environemntal pollution, low production cost, and complete industrial production technology.

Description

Technical field: [0001] The invention relates to bacillus, more specifically to a broad-spectrum bacillus thuringiensis; the invention also relates to a method for preparing an insecticide from the bacillus thuringiensis. [0002] A broad-spectrum bacillus thuringiensis claimed by the present invention has been preserved on June 7, 2002 at the Chinese Type Culture Collection Center of Wuhan University, China, numbering: CCTCC NO: M202025, name: Bacillus thuringiensis Hcy08-02 (Bacillus thuringiensis Hcy08 -02). [0003] Bacillus thuringiensis Hcy08-02 (hereinafter referred to as strain Hcy08-02) was isolated from soil samples and screened out by virulence bioassays using lepidopteran pests such as beet armyworm, cotton bollworm and diamondback moth as test insects. Strains with high virulence to various Lepidoptera pests. It was named Hcy08-02 according to the name of the source of the soil sample where the strain was isolated and the number of the strain. Background techn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00C12NC12N1/20
Inventor 高梅影彭可凡戴顺英
Owner WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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