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Method and system for analysing sample containing multiple fluorescent substance

A sample and component technology, applied in the direction of analyzing materials, fluorescence/phosphorescence, spectrometry/spectrophotometry/monochromator, etc., can solve problems such as complex relationships and achieve high-resolution effects

Inactive Publication Date: 2003-06-18
SHIMADZU SEISAKUSHO CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

And, as mentioned above, because it is a Fourier transform, the relationship between input (spatial information) and output (temporal information) is very complicated

Method used

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  • Method and system for analysing sample containing multiple fluorescent substance
  • Method and system for analysing sample containing multiple fluorescent substance
  • Method and system for analysing sample containing multiple fluorescent substance

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Embodiment Construction

[0080] Figure 6 A fluorescent substance analyzer as an embodiment of the first mode of the present invention is explained. exist Figure 12 Among them, the light source 111 can be, for example, an erbium-doped fiber laser, a mode-locked titanium:sapphire laser or a mode-locked chromium:forsterite laser, which can generate ultrashort optical pulses. The ultrashort light pulses are focused by the first optical system 112 on a point in a transparent substance 113 (such as water or quartz crystal). At this point of convergence, a nonlinear interaction occurs between the substance 113 and the ultrashort light pulse, producing a continuous spectral white light pulse. The continuous spectrum white light pulses enter a second optical system 114 comprising lenses, mirrors and the like. The second optical system 114 irradiates the pulses onto a dispersive element 115 which in turn disperses the pulses into a spectrum. The dispersed light enters a collimator 116 which feeds the ligh...

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PUM

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Abstract

The present invention proposes a method of and system for analyzing samples (particularly, bio-samples), wherein the continuous spectrum white light pulse generated from an ultra-short optical pulse is used in a simple manner. By a system according to the invention, continuous spectrum white light pulses generated by converging ultra-short optical pulses on a point in a transparent substance 23 are dispersed into a spectrum of light by a dispersing element 25, and a plurality of objective wavelength components are separately selected by small reflection mirrors, masked mirrors or the like. The selected objective wavelength components are combined by the dispersing element 25 into a composite optical pulse containing the objective wavelength components. The composite optical pulse is irradiated onto the bio-sample 28 or other samples, whereby only the desired fluorescent substances are excited by the objective wavelength components corresponding to them. The selected objective wavelength components may preferably have different light path lengths. By this construction, the component optical pulses corresponding to the objective wavelengths in the composite optical pulse are separated from each other, so that the noise due to the interference of different wavelength components is prevented.

Description

technical field [0001] The present invention relates to a method and system for analyzing a sample by simultaneously exciting a plurality of fluorescent substances included in the sample. The present invention enables simultaneous and independent tracking of the movement and / or activity of various proteins, etc., included in a living body, since many proteins and other substances included in a living body are naturally fluorescent, or can be made to emit light using fluorescent probes fluorescence. Background technique [0002] When the femtosecond (10 -15 In seconds) The ultra -short light pulse of the class will gather and illuminate the material in the space, and the energy density of the irradiation point becomes very high, which causes linear and non -linear interactions between the light pulse and the material. This interaction produces many phenomena, one of which is the generation of white light pulses with a continuous spectrum (hereinafter referred to as "continu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01J3/10G01J3/44G01N21/01G01N21/64
CPCG01N2021/6419G01J2003/1213G01J3/4406G01J3/0229G01J3/10G01N21/645
Inventor 伊东一良福井希一大久保邦彦
Owner SHIMADZU SEISAKUSHO CO LTD
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