Collectins as adjuvants

A technology of collectin and collection, applied in the field of vaccine compositions, can solve the problem of lack of in-depth research on thymus-independent antigens and the like

Inactive Publication Date: 2004-04-07
詹斯·C·詹斯尼厄斯 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The effect of complement on the response to thymus-independent antigens has not been well studied and available data are conflicting

Method used

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  • Collectins as adjuvants
  • Collectins as adjuvants
  • Collectins as adjuvants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0143] This example shows that individuals with low levels of MBL have lower levels of mature antibodies compared to normal control individuals.

[0144] Materials and methods

[0145] Serum samples: Sera were obtained from 20 adults (aged over 16 years) with homozygous C2 deficiency and 20 healthy blood donors. Selective C2 deficiency in serum determined by rocket electrophoresis and most cases further characterized on a molecular genetic basis 39 . All sera were stored in aliquots at -80°C. An additional control group (n=20) consisted of 19 and healthy people with normal C2.

[0146] Bacterial antigens: Salmonella typhimurium (serotype B: O-4, 12) SH4809 octose polyacrylamide conjugate, Salmonella Thompson (serotype C: O-6, 7) IS40 decyl sugar polypropylene Amide conjugates and Salmonella enteritidis (serotype D: O-9,12) SH 1262 octose polyacrylamide conjugates prepared as described by Chernyak et al. 40 . The prepared conjugates are called BO, CO and DO.

[0147] Bu...

Embodiment 2

[0168] Transgenic mice were created in which one of the two MBL genes, MBL-A and MBL-C, was silenced by homologous recombination. These mice were bred, and double MBL-deficient mice were generated by cross breeding.

[0169] Mice were grouped as follows:

[0170] ● MBL deficiency group

[0171] ● MBL sufficient group (wild type)

[0172] ● MBL-deficient mice reconstituted with MBL

[0173] Each group of mice was immunized with: 1) polysaccharide (mannan purified from yeast) 2) polysaccharide-based pneumococcal vaccine (Pneumovax II); 3) glycoprotein (ovalbumin and / or HbsAg) 4) ovalbumin and / or HbsAg, where sugars have been removed by treatment with glycosidases 5) neoglycoproteins (serum albumin, which has been glycosylated by chemical coupling of sugars) 6) expression of intact glycosylated envelope proteins Enveloped virus (influenza A virus) 7) Influenza A virus grown in the presence of tunicamycin added to the medium to prevent glycosylation.

[0174] Each antigen was...

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Abstract

The present invention provides a vaccine composition comprising a collectin such as mannose binding protein (MBL) and an immunogenic determinant. The invention also describes methods of immunizing individuals with the compositions, and the use of collectins in the preparation of vaccine compositions.

Description

field of invention [0001] The present invention relates to vaccine compositions comprising collectins and immunogenic determinants. Further, the present invention relates to a method of immunizing an individual with said composition, and also relates to the use of collectin in the preparation of a vaccine composition. Background technique [0002] Animals have developed different, complex coping strategies to defend against infection. The immune response can be mainly divided into: adaptive immune response (adaptive immune response) and innate immune response (innate immune response). The adaptive immune response is one in which adaptation occurs and cells play a major role; the innate immune response is one that is readily available and is primarily based on molecules present in body fluids. The innate immune system is functional at birth, while the adaptive immune response acquires its full ability to protect the body in early childhood (Janeway et al., 1999). [0003] ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39C07K14/47
CPCA61K2039/57C07K14/4726A61K2039/55516A61K39/39
Inventor 詹斯·C·詹斯尼厄斯安德斯·肖霍姆
Owner 詹斯·C·詹斯尼厄斯
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