Determination of one section vagina bacillus Gardner DNA sequence (3) specificity and its application

A technology of Gardnerella and DNA sequence is applied in the field of determination of DNA sequence specificity of Gardnerella vaginalis, and the effect of low cost and simple operation is achieved.

Inactive Publication Date: 2004-05-05
昆明寰基生物芯片开发有限公司
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  • Abstract
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Problems solved by technology

[0002] Gardnerella vaginalis (Gardnerella vaginalis) is a pathogen that frequently infects the vagina of women. Accurate diagn

Method used

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  • Determination of one section vagina bacillus Gardner DNA sequence (3) specificity and its application
  • Determination of one section vagina bacillus Gardner DNA sequence (3) specificity and its application
  • Determination of one section vagina bacillus Gardner DNA sequence (3) specificity and its application

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Embodiment Construction

[0019] First, using Gardnerella vaginalis (Gardnerella vaginalis) as the keyword, the nucleotide sequence of Gardnerella vaginalis (Gardnerella vaginalis) was searched in the gene database. A total of several closely related nucleotide sequences were obtained, and these nucleotide sequences were compared with the nucleotide sequences (nr) in the gene database using the Blast online software on the NCBI network to exclude non-specific nucleotide sequences, A 1 Kb specific nucleotide sequence was obtained. More than 20 pairs of PCR primers were designed based on this sequence, DNA was extracted from clinical specimens, PCR amplification was performed, and a pair of sequences and primers suitable for PCR amplification were screened out. The products obtained by PCR amplification were consistent with the expected size (see Fig. 1. No. 1 in the figure is standard molecular weight DNA, and the molecular weights from top to bottom are: 622, 527, 404, 309, 242 / 238, 217, 201, 190, 180,...

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Abstract

The specificity and PCR amplification primer of a viginal Gardener's bacillus DNA sequence (3) fragment are determined by searching and analyzing the data of gene database. Said sequence with 196 bp in length is obtained by PCR amplification and clone of said viginal Gardener's bacillus infected specimen. It can be used to diagnose the viginal Gardener's bacillus with high speed and correctness and low cost.

Description

Technical field: [0001] The invention relates to the specificity determination and application of a DNA sequence (3) of Gardnerella vaginalis (Gardnerella vaginalis) and belongs to the technical field of biomedicine. Background technique: [0002] Gardnerella vaginalis (Gardnerella vaginalis) is a pathogen that frequently infects women's vagina. Accurate diagnosis is the basis for immediate and reliable treatment. At present, it can only be diagnosed according to clinical indications. PCR and gene chip diagnosis is a fast and accurate detection method. Diagnostic methods of this type rely on the availability of specific nucleic acid sequences. Invention content: [0003] The invention is to determine a specific nucleotide sequence of Gardnerella vaginalis (Gardnerella vaginalis) and its PCR primers through bioinformatics and molecular biology techniques. The sequence and corresponding PCR primers can be used to establish a gene chip and PCR method for diagnosis of Gardne...

Claims

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Application Information

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IPC IPC(8): C07H21/04C12Q1/68
Inventor 谭德勇朱宝生
Owner 昆明寰基生物芯片开发有限公司
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