Method for increasing cabbage self-conpatible index treated by quercetin

Abstract

The present invention relates to quercetin treatment process of raising self-compatible index of cabbage. Quercetin solution of 500-1500 micromol/L concentration is sprayed to the bud of self-incompatible cabbage material to block the conducting of self-incompatible signal and overcome the self-incompatibility of cabbage. The said method is established on the basis of molecular mechanism, so that it has the features of less consumption, sensitive reaction, etc. The present invention has greatly raised self-compatible index or even conversion to self-compatible line.

Description

technical field [0001] The invention relates to the technical field of vegetable breeding, in particular to a method for treating cabbage self-incompatibility lines with quercetin to improve the self-compatibility index. Background technique [0002] Brassica oleracea L. has obvious heterosis and is widely used in production. Its hybrid seeds are mainly obtained by mating between self-incompatibility (SI) lines of cabbage. One of the main problems encountered in preparing cabbage hybrids with self-incompatible lines is that pollination at the bud stage is required for breeding self-incompatible lines, which is time-consuming and labor-intensive, which greatly increases the cost of seed production. In order to improve the self-compatibility during pollination, so as to facilitate the reproduction and preservation of self-incompatible lines, some researches have been carried out at home and abroad. Throughout the published research reports and literature, there are more than...

AI Technical Summary

Problems solved by technology

After the separation and identification of two genes SLG (S-Locus Glycoprotein) and SRK genes that determine the self-incompatibility response at the S locus of the cabbage stigma, the mechanism of self-incompatibility in Brassica plants is considered to be pollen After the ligand in the granule binds to the complex of protein kinases SRK and SLG, the conformation of the complex is changed, and the intracellular Ser / Thr protein kinase domain of SRK catalyzes the protein phosphorylation / dephosphorylation reaction, thereby turning The extracellular signal is transmitted into the cell, causing a series of cellular responses, and finally inhibiting pollen germination, resulting in self-incompatibility

Although the research on the molecular mechanism of cabbage self-incompatibility provides the possibility to overcome the self-incompatibility of cabbage at the molecular level, there is no research report on the method in this regard