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Compound bacterium agent capable of improving various properties of animals and application of compound bacterium agent

A compound bacterial agent, animal technology, applied in the application, animal feed, animal feed and other directions, can solve the problems of soil and water pollution, large volume, production and life hazards and other problems

Inactive Publication Date: 2016-10-26
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the characteristics of large volume and large output of traditional Chinese medicine dregs, traditional Chinese medicine dregs are mainly piled up. Stacking traditional Chinese medicine dregs not only wastes the active ingredients remaining in the dregs, but also causes waste of resources. Pollution of water and soil resources has brought serious harm to people's production and life

Method used

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  • Compound bacterium agent capable of improving various properties of animals and application of compound bacterium agent
  • Compound bacterium agent capable of improving various properties of animals and application of compound bacterium agent
  • Compound bacterium agent capable of improving various properties of animals and application of compound bacterium agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Embodiment 1, the preparation of probiotics for animals

[0055] 1. Preparation of compound microbial agent for animals

[0056] Pick an appropriate amount of Aspergillus niger ACCC 30583 spores from the PDA medium, and dilute them to 1×10 with sterile water. 8 The suspension of CFU / mL was inoculated with PD medium according to 1% inoculation amount and carried out shaking flask culture, 160rpm, 28°C for 5 days, and the content of Aspergillus niger ACCC 30583 was 10 13 CFU / mL of Aspergillus niger ACCC 30583 fermentation broth.

[0057] Pick the colony of Bacillus subtilis ACCC 03291 from LB medium and dilute it to 1×10 with sterile water 8 The suspension of CFU / mL was inoculated with LB liquid medium according to 1% inoculum and carried out shaking flask culture, 160rpm, 37°C for 3 days, and the content of Bacillus subtilis ACCC 03291 was 10 12 CFU / mL of Bacillus subtilis ACCC 03291 fermentation broth.

[0058] The aspergillus niger ACCC 30583 fermented liquid and t...

Embodiment 2

[0061] Embodiment 2, utilize probiotics for animals to improve the growth performance of pigs

[0062] 1. Pig ration

[0063] This example provides four kinds of pig fattening diets, which are basal diet (Table 1), 1% astragalus dregs diet, 0.75% animal probiotics diet and 1% animal probiotics diet. The 1% astragalus dregs diet is a diet obtained by adding 1% astragalus dregs to the basic diet, and the 0.75% animal microecological preparation diet is added to the basic diet. The ration obtained by the animal probiotic preparation of 0.75% of the grain quality, 1% of the animal probiotic preparation ration is to add the animal of embodiment 1 that is 1% of the basal ration quality in the basal ration Diets obtained with probiotics.

[0064] Table 1. Raw material composition and nutritional level of pig basal diet

[0065]

[0066]

[0067] 11.58 g of premix provides per kg of diet: vitamin A 4000IU, vitamin D 3 1250IU, Vitamin B 2 1mg, vitamin E 50IU, vitamin K 3 ...

Embodiment 3

[0096] Embodiment 3, utilize probiotics for animals to improve the growth performance and immune performance of chickens

[0097] 1. Chicken diet

[0098] This example provides four kinds of diets for chicks, which are the basic diet for the brooding period, the basic diet for the rearing period, the diet with 4% astragalus dregs and the diet with 4% microecological preparations for animals. The composition of the basal ration in the brooding period and the basal ration in the growing period is shown in Table 5. The 4% astragalus dregs diet is a diet obtained by adding 4% of astragalus dregs to the basic diet in the growing period, and the 4% animal microecological preparation diet is obtained by adding 4% of the basic diet in the growing period. Add the diet obtained from the probiotic preparation for animals in Example 1, which is 4% of the mass of the basal diet in the growing period.

[0099] Table 5. Raw material composition and nutritional level of chicken basal diet ...

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Abstract

The invention discloses a compound bacterium agent capable of improving growth properties of animals and the application of the compound bacterium agent. Active components of the compound bacterium agent provided by the invention consist of bacillus subtilis and aspergillus niger. Experiments prove that a microecological preparation for animals, obtained by culturing the compound bacterium agent through fermentation culture mediums is capable of increasing the daily gain of chickens, reducing the feed conversion rate of the chickens, increasing the content of total superoxide dismutase in chicken serum, increasing the thymus index of chickens, increasing the spleen index of the chickens, increasing the bursa fabricii index of the chickens, increasing the content of newcastle disease antibodies in the chicken serum, increasing the content of total protein in the chicken serum, and increasing the content of albumin in the chicken serum.

Description

technical field [0001] The invention relates to a compound microbial agent for improving various performances of animals and its application. Background technique [0002] Probioties, also called live bacteria preparations (Bigone) or biobiotics, refers to the use of microecological principles, the use of probiotics or probiotic growth-promoting substances that are beneficial and harmless to the host, and are made by special processes preparations. [0003] Probiotics are green and safe additives that have no drug residues, do not cause environmental hazards, and do not pose real or potential threats to product quality and safety and human living environment. It is one of the development directions of safe feed additives. [0004] Traditional Chinese medicine dregs are residues after extraction of active ingredients from traditional Chinese medicinal materials, and are generally treated as waste in the extraction industry of traditional Chinese medicine. At present, the in...

Claims

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Application Information

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IPC IPC(8): A23K50/75A23K10/18A01K67/02A23K10/37A23K10/10
CPCA01K67/02Y02P60/87
Inventor 韩春杨顾金刚张瑞颖陈华刘峰刘翠艳龚明波李世贵
Owner ANHUI AGRICULTURAL UNIVERSITY
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