Fast disclosing solution for lymph node examination and usage thereof

A lymph node and rapid technology, applied in the field of lymph node examination of malignant tumor specimens, can solve the problems of time-consuming, expensive, laborious and cost-intensive, and achieve the effect of easy selection and simple operation.

Inactive Publication Date: 2005-01-26
CENT SOUTH UNIV
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Problems solved by technology

[0004] 1. The manual method commonly used in clinical practice is to find and pick lymph nodes on specimens by visual inspection and hand touch, which is simple and easy to implement, but it is easy to miss metastatic lymph nodes with a diameter of less than 5 mm, and these missed metastatic lymph nodes account for 50% of all metastatic lymph nodes. %, so it may cause inaccurate diagnosis and t

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  • Fast disclosing solution for lymph node examination and usage thereof

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Embodiment Construction

[0037] 1. Materials and Methods

[0038] 1.1 Materials From June to December 2001, there were 20 specimens collected from the Department of General Surgery of the Third Xiangya Hospital of Central South University after radical resection of colorectal cancer, including 11 males and 9 females. Age ranged from 38 to 68 years, with an average of 55.35 years; 13 cases of colon cancer and 7 cases of rectal cancer; all colorectal cancers underwent complete surgical resection of the primary tumor and other mesentery.

[0039] The composition of quick display liquid is by volume percentage: 95% absolute alcohol 55%, ether 20%, turpentine 10%, glacial acetic acid 5%, buffered formalin 10%, sodium dihydrogen phosphate 5g, methylene blue powder 2g and mix well , prepare 1000ml, and put it in a well-sealed container for later use.

[0040] 1.2 Methods Using the self-control method, each specimen was processed separately by the traditional method and the rapid display liquid method.

[0...

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Abstract

A kind of quick-speed showing liquid comprises (percentage by volume) absolute alcohol, aether turpentine and monosodium orthophosphate and little methylene blue. The usage is that one should put malignant tumour specimen immobilizing by 10 percents formalin into a hermetization pot full of quick-speed showing liquid of lymph gland checking and immerse it for 6-12 hours. The volume of liquid dosage is the third times of the specimen. This invention not only increases detecting number of normal and metastasizing lymph gland greatly, but also makes metastasizing rate (the percent of metastasizing case load and total case load) exacter and checks it faster, cheaper and more veracious.

Description

technical field [0001] The invention relates to lymph node inspection of malignant tumor specimens, especially a liquid for inspection. [0002] Clinically, all lymph nodes must be detected in the resection specimens of malignant tumors, so as to further check whether there is lymphatic metastasis and the degree of metastasis, so as to guide diagnosis, treatment and prognosis. Taking colorectal cancer as an example, the international standard for the minimum number of lymph nodes to be removed per specimen is 8-16. Background technique [0003] The current methods for lymph node examination of resected specimens of malignant tumors include: [0004] 1. The manual method commonly used in clinical practice is to find and pick lymph nodes on specimens by visual inspection and hand touch, which is simple and easy to implement, but it is easy to miss metastatic lymph nodes with a diameter of less than 5 mm, and these missed metastatic lymph nodes account for 50% of all metastati...

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Application Information

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IPC IPC(8): G01N33/483G01N33/50G01N33/574
Inventor 陈道瑾向开敏
Owner CENT SOUTH UNIV
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