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Relativity between synthetase of endothelium-nitrogen oxide and essential hypertension

A nitric oxide, gene technology, applied in the fields of molecular biology and medicine, can solve the reports that do not confirm the correlation between NOS gene SNP and essential hypertension, and do not confirm the correlation between NOS gene and essential hypertension, etc. question

Inactive Publication Date: 2005-08-31
FUDAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Although there have been many studies on the relationship between various gene polymorphisms and essential hypertension, there is no report on the correlation between NOS gene and essential hypertension, let alone the correlation between the SNP of NOS gene and essential hypertension sex reports

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] 1.1 Research object

[0068] Since blood pressure is a quantitative trait that is affected by multiple genes and is affected by various environmental factors, it is necessary to consider the issue of genetic heterogeneity when analyzing related genes. Selecting isolated populations with a relatively single genetic background as materials for the study of polygenic diseases will help reduce the impact of genetic heterogeneity. reduce. Selecting such a group, whether it is used for linkage disequilibrium analysis or selecting families for family analysis, will help to improve the detection sensitivity of hypertension susceptibility loci. Therefore, in this embodiment, the isolated population is selected as the research object (the isolated population has the advantages of relative consistency of genetic background and relatively small number of founders).

[0069] On the basis of informed consent, 324 individuals of Han nationality over the age of 50 were randomly colle...

Embodiment 2

[0088] Essential Hypertension Susceptibility Detection Kit

[0089] As described in Example 1, the T→G mutation at position 20455 in SEQ ID NO: 1 is closely related to hypertension. Therefore, NOS gene-specific primers can be designed based on this mutation and then amplified using the patient's DNA as a template for detection.

[0090] name

sequence

concentration

sense primer

SEQ ID NO: 2

100pmol

antisense primer

SEQ ID NO: 3

100pmol

PCR reaction solution

Containing Taq Enzyme dNTP Magnesium Ion PCR Reaction Buffer

[0091] Take 3ml of blood from the male patient to be tested, and use a conventional method (or use a specific kit) to extract DNA from the blood. Dilute the PCR primers in the hypertension detection kit to lìmol / ìl, and use the extracted DNA as a template to perform a PCR reaction with the provided primers. After purification of PCR products, ABI-PRISM TM 377 DNA sequencer was used for two-way se...

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Abstract

A process for testing the susceptibility of primary hypertension includes detecting if there are variations in hypophloeodal NO synthetase gene NOS, transcript and / or protein of an individual and determining its high susceptibility if there are. Its reagent kit is also disclosed.

Description

technical field [0001] The present invention relates to the fields of molecular biology and medicine. More specifically, it relates to the single nucleotide polymorphism (singlenucleotide polymorphism, SNP) of the endothelial nitric oxide synthase gene (endothelial nitric oxide synthase, NOS) and its correlation with essential hypertension. The present invention also relates to methods and kits for detecting these SNPs. Background technique [0002] Hypertension refers to a group of clinical syndromes with elevated systolic or diastolic blood pressure. There is an obvious causal relationship between the increase of blood pressure and the occurrence of coronary heart disease, renal dysfunction, hypertensive heart disease and hypertension complicated with stroke. The latest diagnostic criteria for hypertension are systolic blood pressure ≥ 19kpa (140mmHg) or diastolic blood pressure ≥ 12kpa (90mmHg), and those who meet one of them can be diagnosed as hypertension. [0003] ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N33/68
Inventor 金力黄薇姜正文王颖张晨辉李艳平王志敏肖君华卢大儒
Owner FUDAN UNIV
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