Method for inductively producing snow lotus somatic embryo

A technology of snow lotus and selection of snow lotus, applied in the field of bioengineering, can solve problems such as endangered, natural resources of snow lotus cannot reproduce, etc.

Inactive Publication Date: 2006-02-22
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in recent years, with the continuous development of the medicinal effect of snow lotus, people have paid more and more attention to it. The phenomenon of illegal digging is v

Method used

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  • Method for inductively producing snow lotus somatic embryo
  • Method for inductively producing snow lotus somatic embryo
  • Method for inductively producing snow lotus somatic embryo

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] callus induction

[0018] a. Select 200 Xinjiang snow lotus seeds, first sterilize the surface with 75% alcohol for 20 seconds, then soak in 0.1% mercuric chloride solution for 3 minutes, then rinse with sterile water until clean, put them on the culture medium of a sterile culture dish, 8 The daily germination rate reaches 70%;

[0019] b. Take the leaves of just germinated seedlings as explants, cut into 0.5cm in size 2 , put it on the callus induction medium of agar-solidified MS+2,4-D0.05mg / L+BA0.5mg / L, the inoculum size is 250ml triangular flask containing 50ml agar-solidified medium, and then 5 explants Body, solid culture conditions are: fluorescent lamp 2500lx, light 12h / d, temperature 25±1°C, humidity 65±5%, after 20-25 days, callus is obtained; solid medium is added with 3% sucrose, and the pH is adjusted to 5.8 , the sterilization time is 20min, and the temperature is 121℃;

[0020] induction of somatic embryos ...

Embodiment 2

[0023] callus induction

[0024] a. Select 300 Xinjiang snow lotus seeds, first sterilize the surface with 75% alcohol for 25 seconds, then soak in 0.1% mercury chloride solution for 4 minutes, then rinse with sterile water for 3 times, put them on MS medium, and the germination rate after 11 days reach 80%;

[0025] b. Take the leaves of just germinated seedlings as explants, cut into 0.6cm in size 2 , put it into agar-solidified MS+2,4-D0.1mg / L+BA1.0mg / L callus induction medium, 250ml Erlenmeyer flask containing 50ml agar solidified medium, connect 6 explants, solid The culture conditions are as follows: fluorescent lamp 3500lux, light 12h / d, temperature 25±1°C, humidity 65±5%, after 23 days, callus is obtained; solid medium is added with 4% sucrose, pH is adjusted to 5.85, and the sterilization time is uniform. for 20 minutes, and the temperature was 121°C.

[0026] induction of somatic embryos

[0027] c. Then subculture once on t...

Embodiment 3

[0029] callus induction

[0030] a. Select 400 snow lotus seeds, first sterilize the surface with 75% alcohol for 30 seconds, then soak in 0.1% mercuric solution for 5 minutes, then rinse with sterile water for 3 times, put them on a sterile petri dish, and the germination rate after 15 days reach 90%;

[0031] b. Take the leaves of just germinated seedlings as explants, cut into 0.8cm in size 2 , put it into agar-solidified MS+2,4-D2.0mg / L+BA3.0mg / L callus induction medium, 250ml Erlenmeyer flask containing 50ml agar solidified medium, connect 6 explants, solid The culture conditions are: fluorescent lamp 3500 lux, light 12h / d, temperature 25±1°C, humidity 65±5%, after 25 days, callus is obtained; solid medium is added with 5% sucrose, pH is adjusted to 5.9, and the sterilization time is uniform. for 20 minutes, and the temperature was 121°C.

[0032] induction of somatic embryos

[0033] c. Then subculture once on the medium of MS...

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Abstract

The invention relates to a method for inducing the body embryo of saussurea involucrate, which is: inducing the callus of saussurea involucrate seeds, and getting the body embryo of saussurea involucrate by the body embryo induction. According the leaflet regenerating principle, taking seedling cotyledons as explants, then inducing them by solid and liquid suspension culturing to get the body embryo of saussurea involucrate, laying the foundation of producing artificial seeds.

Description

technical field [0001] The invention belongs to the technical field of bioengineering and relates to plant tissue culture technology, in particular to the induction of somatic embryos of Xinjiang snow lotus. Background technique [0002] Xinjiang Snow Lotus (Saussurea Involucrata Kar.et Kir.ex Maxim), a species of Saussurea D C in the Compositae family, has multiple functions such as promoting menstruation and blood circulation, warming the palace and dispelling blood stasis, dispelling cold and dehumidification, and strengthening tendons and yang. The medicinal plants mainly grow in alpine meadows, alpine ice debt stones, flowstone beach rock crevices, cliff rock crevices, etc. at an altitude of 2400-4100m. It is one of the rare plant species in Xinjiang and has high medicinal value. value. However, in recent years, with the continuous development of the medicinal effect of snow lotus, people have paid more and more attention to it. The phenomenon o...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 林侃王晓军赵民安刘敏努尔波拉提赵海清贾莉芳付小春
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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