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Method for overcoming self-incompatibility of brassica plant

A Brassica plant, affinity technology, applied in the direction of botanical equipment and methods, plant phenotype improvement, application, etc., to achieve the effect of favorable germination, convenient operation, and high efficiency

Inactive Publication Date: 2006-03-22
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The use of carbonic anhydrase to overcome self-incompatibility in Brassica plants has not been reported

Method used

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  • Method for overcoming self-incompatibility of brassica plant

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Comparison scheme
Effect test

Embodiment 1

[0017] When the cabbage self-incompatibility line material enters the initial flowering stage, spray 2 μg / ml carbonic anhydrase solution on the flowers that have just emerged from the stigma at 9:00 to 10:00 in the morning until fog droplets can be seen on the stigma. Spray once every 1 day, and stop spraying when 90% of the flowers on the plant are in bloom. The average pod setting rate of self-incompatible cabbage lines under this treatment can reach 39.8%, the average number of seeds per pod is 5, and the self-compatibility index increases from 0.08 to 1.99. The seeds are fuller.

Embodiment 2

[0019] When the cabbage self-incompatibility line material enters the initial flowering period, spray 8 μg / ml carbonic anhydrase solution on the flowers that have just emerged from the stigma at 9:30 to 10:30 in the morning, until fog droplets can be seen on the stigma, Spray once every 1 day, and stop spraying when 90% of the flowers on the plant are in bloom. The average pod setting rate of the cabbage self-incompatibility line under this treatment can reach 52.5%, the average number of seeds per pod is 7, and the self-compatibility index increases from 0.08 to 3.68. The seeds are fuller.

Embodiment 3

[0021] When the cabbage self-incompatibility line material enters the initial flowering period, spray 4 μg / ml carbonic anhydrase solution on the flowers that have just emerged from the stigma at 9:00 to 10:00 in the morning, until the stigma can be seen. Spray once every 1 day, and stop spraying when 90% of the flowers on the plant are in bloom. The average pod setting rate of self-incompatible lines of cabbage under this treatment can reach 78.4%, the average number of seeds per pod is 4, and the self-compatibility index increases from 0.31 to 3.12. The seeds are fuller.

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Abstract

The present invention relates to the breeding technology for brassica plant, and especially carbonic anhydrase treatment method of overcoming self-incompatibility of brassica plant. Specifically, carbonic anhydrase solution of 2-10 microgram / ml concentration is sprayed to the blooms with just emerged stigmata in 8:30-10:30 AM every other day in the initial blooming period until blooming in 90%. The sprayed carbonic anhydrase solution is preferably in atomized form for high absorption. The said method may be used in Brassica campestris, Brassica oleracea, Brassica napus and Brassica pekenensis to raise the self-compatibility index in different degree.

Description

technical field [0001] The invention relates to the technical field of Brassica plant breeding, in particular to a method for overcoming the self-incompatibility of Brassica plants by treating with carbonic anhydrase. Background technique [0002] Self-incompatibility is a common phenomenon in the plant world. It is commonly found in monoecious plants. It is a reproductive isolation that is beneficial to cross-pollination formed during the long-term evolution of plants. Self-incompatibility means that the pollen of the white flower cannot germinate on its own stigma, or cannot penetrate the stigma after germination, or the pollen tube cannot continue to extend in the style after penetrating the stigma, or the pollen tube reaches the embryo sac. Egg cells cannot be combined, so the pollen and pistil show mutual inhibition. Self-incompatibility has important theoretical and practical significance in the reproductive biology of plants and the utilization of heterosis in crops....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H3/04
Inventor 吴沿友李萍萍毛罕平管荣展
Owner JIANGSU UNIV
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