Method for breeding clonal seedling by utilizing muskmelon seed leaf segment

Abstract

The method for propagating clonal seedling by utilizing cotyledonary node of sweet melon includes the following procelures: (1), selecting material and sterilization; (2), preculture; (3), starting culture; (4), adventitious bud induction and elongation culture; (5), successive transfer culture; (6), rooting culture; and (7), regenerated plant naturalization. Said invention also provides the concrete steps of the above-mentioned every procedure.

Description

technical field [0001] The invention relates to a method in the field of agricultural technology, in particular to a method for propagating cloned seedlings by using cotyledonary nodes of muskmelon. Background technique [0002] The first generation of melon hybrids has obvious heterosis, and the yield is generally 20%-99% higher than that of the parent variety, even up to 300%. Moreover, the sugar content, dry matter content and storage ability of the fruit are usually improved. In the cultivation of melons in Japan, it is basically the first generation of hybrids. The production and scientific research of muskmelon in my country has paid attention to this problem in the mid-1980s. In recent years, the main production areas have used the first generation of hybrids in production. In short, the utilization of the first generation of melon hybrids has become a new trend in the production of melons in the world. Although the advantages of hybrids are obvious, the production ...

AI Technical Summary

Problems solved by technology

But this method still has some difficult problems

The emergence of "buds" can be seen after about 10 days of explant culture, and 30-50 "buds" can appear on the surface of explants after another 10 days of culture. Produces relatively few elongatable shoots, resulting in a final reproductive coefficient that is too low to be practical for production