Expression plasmid for rep gene, P5NIE cisform element fixed point integration system and its preparation method and uses

Abstract

This invention belongs to the biology technique and gene treatment technique field, mainly to a Rep gene expression grain, P5NIE serial element fixing conformity system and it's preparing method and its application in the gene treatment. And this system is composed by the expression grain and rep gene expression grain, wherein the EGFP reporting gene has the P5NIE serial element and EGFP reporting gene. And the system can be used in the external gene importing of the human cell, and the programme of the preparation is simple, P5NIE serial element combining with other reporting genes can achieve the aim of conforming the external gene and long expression, and this invention can be used in the gene treatment for a polarity of diseases combining with other therapy gene.

Description

technical field [0001] The invention belongs to the technical field of biotechnology and gene therapy, and specifically relates to a rep gene expression plasmid, a P5NIE cis-element fixed-point integration system and a preparation method and application thereof. Background technique [0002] Gene therapy has advantages that cannot be replaced by other methods for the treatment of genetic diseases, but gene therapy needs to modify the patient's genome, which makes people worry about its safety. [0003] There are currently several gene manipulation transfer pathways. Viral vectors have high gene transfer efficiency, but there are many deficiencies in safety: such as adenovirus, adeno-associated virus, retroviral vectors, and lentiviral vectors, which can be randomly integrated into the host genome causing insertion mutation. The gene transfer efficiency of non-viral vectors is low, the expression of foreign genes is short-lived, and there are also safety problems of random i...

AI Technical Summary

Problems solved by technology

[0002] Gene therapy has irreplaceable advantages for the treatment of genetic diseases, but gene therapy needs to modify the patient's genome, which makes people worry about its safety

[0003] There are currently several gene manipulation transfer pathways. Viral vectors have high gene transfer efficiency, but there are many deficiencies in safety: such as adenovirus, adeno-associated virus, retroviral vectors, and lentiviral vectors, which can be randomly integrated into the host genome causing insertion mutation

Since the C in the second GAGC box of P5RBE, the core region related to integration in P5IEE, has changed to T, and there are also many base changes in the first and fourth GAGC boxes, which are different from the RBE at the AAVS1 site of human chromosome 19 Compared with only 62.5% homology, the binding ability of P5RBE and Rep protein will be greatly reduced, which will also affect the efficiency of P5IEE as an integrated cis-element

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