Marine bacteria strain for producing prodigiosin and the method for producing prodigiosin

A technology for the production of prodigiosin and marine bacteria, applied in the field of marine bacterial strains, can solve problems such as difficult mass production and research impact, and achieve the effects of low toxic and side effects, fast reproduction, and strong immunosuppressive activity

Inactive Publication Date: 2006-12-27
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, prodigiosin is usually obtained by fermenting and purifying its metabolites with Serratia as the production strain. Due to its preparation process, it is difficult to produce in large quantities, which will affect further research.

Method used

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  • Marine bacteria strain for producing prodigiosin and the method for producing prodigiosin

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Embodiment Construction

[0025] The method for producing prodigiosin using Pseudomonas sp.105001 CCTCC M 205164 as a production strain will be further described in detail below.

[0026] The specific steps for producing prodigiosin with Pseudomonas sp.105001 CCTCC M 205164 are as follows:

[0027](1) Mother seed preparation: produce fermented yeast species with 2216E liquid medium, the formula of described 2216E liquid medium is: peptone 5g, yeast extract 1g, ferric citrate 0.1g, aged seawater 1000ml, pH7.6;

[0028] (2) Inoculate the mother species in the fermentation medium for fermentation and production: the fermentation production conditions are aeration culture, growth temperature: 25-30°C, the formula and preparation of the fermentation medium are as follows: organic component A: in 10g Add 0.1 g of hydrolase to fish meal, hydrolyze at 60°C for 4 hours, and take the supernatant; Inorganic component B: coarse salt 32 g, KCl 0.644 g, NaHCO 3 0.192g, KBr 0.096g, H 3 BO 3 0.026g, SrCl 2 0.02...

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Abstract

The invention provides a marine bacterial strain screening from the marine bacteria which can produce prodigiosin and a method for fermentation production of prodigiosin by using the said strain. The said marine bacterial strain is Pseudomonas sp.105001 CCTCC M 205164. The red metabolite of the said strain - the prodigiosin compound of the invented product can be prepared by selecting Pseudomonas sp.105001 CCTCC M 205164 as production strain, preparing mother seeds, by fermentation culture and extracting red metabolite and purifying metabolite. The new marine bacterial strain provided by this invention is of stable heredity and quick reproduction, which can perform large scale fermentation production and the prodigiosin production using the said marine bacteria, opens a new approach for study and exploitation of marine microbial resource.

Description

technical field [0001] The invention relates to a marine bacterial strain capable of producing prodigiosin screened from marine bacteria and a method for fermenting and producing prodigiosin with the bacterial strain. Background technique [0002] Prodigiosin, with a unique structure and a pyrrole pyrromethene skeleton, was previously mainly derived from the clinical pathogenic microorganism Serratia marcesens. Prodigiosin compounds are a general term for compounds with pyrrole pyrromethene structure characteristics. There may be 2, 3 or 4 pyrrole rings in the entire molecule, and the pyrrole rings may be connected with long chain alkyl groups or other functional groups. Prodigiosin has a variety of significant pharmacological activities: 1) anti-tumor activity; 2) anti-microbial activity; 3) anti-malarial; 4) promotion of cell apoptosis; 5) strong immunosuppressive activity, its immune mechanism and It is related to its unique pyrrole pyrromethene skeleton structure. Due ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P1/04C12P17/16C12R1/38
Inventor 蔡创华周毅频沈鹤琴张穗
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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