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Culture method of Armillaria Staude fungus

A culture method, the technology of Armillaria acuminata, which is applied in the direction of fungi, etc., can solve the problems of difficult separation of medicinal components, long solid-state fermentation period, and large investment

Active Publication Date: 2007-03-07
合肥诚志生物制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The solid-state fermentation cycle is too long, easy to infect bacteria, and difficult to separate effective ingredients; liquid submerged fermentation, although the time is short, requires certain equipment and large investment, it is difficult to achieve in a short time, and the biomass and active substance content of fermentation mycelia are low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0054] (1), the preparation of liquid culture medium:

[0055] Now take the preparation of 1000ml liquid medium as an example, and the non-limiting examples are described as follows:

[0056] 1. Take 8g each of corn flour and soybean flour, add 100ml of water to boil for 20-30 minutes, separate, and soak in this way for three times to get 300ml of soaking liquid, and combine the soaking liquid to 600ml. 2 PO 4 0.5g, MgSO 4 ·7H 2 O 0.3g was added into the infusion liquid, stirred and dissolved, and the volume was adjusted to 1000ml, and sterilized for later use.

[0057] 2. Take 12g corn flour, 12g soybean flour, 12g glucose, 25g sucrose, KH 2 PO 4 1.5g, MgSO 4 ·7H 2 O1g, operation is with embodiment 1.

[0058] 3. Take 8g of corn flour, 12g of soybean flour, 8g of glucose, 25g of sucrose, KH 2 PO 4 0.5g, MgSO 4 ·7H 2 O1g, operation is with embodiment 1.

[0059] 4. Take 12g corn flour, 8g soybean flour, 12g glucose, 15g sucrose, KH 2 PO 4 1.5g, MgSO 4 ·7H ...

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PUM

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Abstract

The invention discloses a culturing method of little halimasch fungi, which comprise the following steps: adopting Armilariella tabescens and halimasch as starting bacteria; culturing seed to obtain solid bacterial suspension in the liquid culture medium; stewing under 24-28 deg.c for 16-22d; extracting active polysaccharide as raw material of drug.

Description

1. Technical field [0001] The invention relates to a method for cultivating microorganisms, in particular to a method for cultivating medicinal fungi, more precisely, a method for cultivating Armillaria agaricus fungi. 2. Background technology [0002] Bright bacteria (Armillariella tabescens) and Armillariella (Armillariella mellea) belong to Fungi Kingdom, Basidiomycota, Laminomycetes, Agaricaceae, White Mushrooms, Armillaria genus. Armillaria minor, also known as false Armillaria, mainly has two fungi under the genus Leucobacteria and Armillaria, both of which are medicinal fungi. [0003] Bright bacteria are mainly used in the treatment of acute and chronic hepatitis, acute cholecystitis, acute exacerbation of chronic cholecystitis, other biliary tract diseases complicated with acute infection, chronic gastritis, and can also be used for radiation protection, anti-tumor, enhancing human immunity, treatment of radiotherapy, chemotherapy Leukopenia, infantile jaundice, ac...

Claims

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Application Information

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IPC IPC(8): C12N1/14
Inventor 沈业寿马金宝郑媛李赓李峰
Owner 合肥诚志生物制药有限公司
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