Method for tissue-culture and induction of adventitous root of pseudo-ginseng

A tissue culture and Panax notoginseng technology, applied in the field of medicine, can solve the problems of slow growth, no method for establishing a liquid culture system of adventitious roots, endophytic fungus contamination, etc., and achieves the effects of rapid growth, simple induction method and high efficiency.

Inactive Publication Date: 2007-04-18
SHANGHAI JIAO TONG UNIV
View PDF1 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this patent-applied technology only involves the roots of Panax notoginseng, and does not involve the induction of adventitious roots from callus from the flower buds, leaves, petioles, stems and fibrous roots of field cultivated Panax notoginseng, nor does it involve the direct induction of regenerated seedlings of Panax notoginseng The method of adventitious roots, but the i

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for tissue-culture and induction of adventitous root of pseudo-ginseng
  • Method for tissue-culture and induction of adventitous root of pseudo-ginseng
  • Method for tissue-culture and induction of adventitous root of pseudo-ginseng

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Induction of Panax notoginseng adventitious root in vitro and establishment of liquid culture system

[0024] Take the callus pieces induced from the stem explants of Panax notoginseng, place them on MS (containing 10mmol / L ammonium nitrate) solid medium supplemented with 2mg / L indolebutyric acid and 0.7mg / L kinetin, and place them at 25°C Adventitious roots formed after 20 days of induction under sterile light-proof conditions. Take the above-mentioned induced adventitious roots, transfer them together with the parental callus to MS liquid medium containing the same composition, and continuously subculture for 3 generations at 26°C with 28 days / generation interval (shaking table speed 100r / min). Adventitious roots were cultured separately from parent tissues. Take the isolated and cultured adventitious roots and transfer them to MS liquid medium containing 5mg / L indole butyric acid, and culture them in the dark at 80r / min at 25°C for 35 days to obtain the a...

Embodiment 2

[0026] Example 2: In vitro induction of Panax notoginseng adventitious root and establishment of liquid culture system

[0027] Take the callus pieces induced from the leaf explants of Panax notoginseng, place them in solid MS (containing 10mmol / L ammonium nitrate) containing 0.1mg / L indolebutyric acid, 5mg / L naphthaleneacetic acid and 0.1mg / L kinetin On the culture medium, adventitious roots formed after being induced for 35 days at 20°C under sterile and dark conditions. Take the adventitious roots induced above, and transfer them together with the parental callus to the MS liquid medium containing the same composition, and continuously subculture at 20°C for 4 generations at an interval of 21 days / generation (shaking table speed 120r / min). Adventitious roots were cultured separately from parent tissues. Take the isolated and cultured adventitious roots and transfer them to MS liquid medium containing 5mg / L naphthaleneacetic acid, and culture them in the dark at 120r / min at...

Embodiment 3

[0029] Example 3: In vitro induction of Panax notoginseng adventitious root and establishment of liquid culture system

[0030] Take the callus pieces induced from the explants of the petiole of Panax notoginseng, put them on the solid medium of MS (containing 5 mmol / L ammonium nitrate) containing 10 mg / L indolebutyric acid and 0.1 mg / L kinetin, at 25 °C Adventitious roots formed after 35 days of induction under sterile and dark conditions. Take the adventitious roots induced above, and transfer them together with the parental callus to the MS liquid medium containing the same composition, and continuously subculture at 26°C for 5 generations at an interval of 35 days / generation (shaking table speed 80r / min). Adventitious roots were cultured separately from parent tissues. The adventitious roots that were isolated and cultured were transferred to MS liquid medium containing 0.1mg / L indolebutyric acid and 5mg / L naphthaleneacetic acid, and cultured in the dark at 120r / min at 25...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

A method for inducing the adventitious root of notoginseng and its tissue culture includes such steps as taking the callus and explant of notoginseng, putting them on the fixing culture medium MS containing one or more of indole-butanoic acid, naphthylacetic acid and kinetin, inducing adventitious root in aseptic and dark condition, transferring the induced adventitious root and its mother tissue the liquid MS culture medium, culturing, separating the adventitious root from its mother tissue, and culturing the adventitious root in liquid MS culture medium containing indole-butanoic acid and/or naphthylacetic acid.

Description

technical field [0001] The invention relates to a method in the technical field of medicines, in particular to a method for inducing and tissue-culturing Radix Notoginseng adventitious roots. Background technique [0002] Panax notoginseng is one of the unique, precious and most commonly used Chinese herbal medicines in my country. It has the functions of dispelling blood stasis, stopping bleeding, reducing swelling and relieving pain. Important raw materials for health care products and medicines, such as Xuesaitong, Yunnan Baiyao, Compound Danshen Dripping Pills and Compound Sanqitongluo Tablets, etc. However, due to the limitation of its growth by geographical conditions and the influence of "cultivated land cannot be reused", the cultivated area is very limited, resulting in a shortage of medicinal materials, which cannot meet the needs of the medical care market. In order to solve the problem of shortage of land resources and non-reuse of land resources, it is urgent to...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H4/00C12N5/04
Inventor 贾伟高先富邱明丰赵爱华梁梦奇谢国祥
Owner SHANGHAI JIAO TONG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap