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Usage of caffeine and its analogue in resistance of hepatitis B replication and use thereof in preparation of medicament for treating viral hepatitis

A hepatitis B virus and analog technology, applied in the field of medicine, can solve the problems of elevated HBV DNA level, not recommended use, and virus resistance mutation

Inactive Publication Date: 2007-05-30
INST OF BIOENG ACAD OF MILITARY MEDICAL SCI OF THE CHINESE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

), its effective rate is 30-60%, but it is not recommended for patients with few liver cells such as severe hepatitis
Another class of antiviral drugs is nucleoside analogs, which mainly target viral reverse transcriptase. Lamefluridine, which is currently undergoing clinical phase I and II trials, is effective for immune-tolerant HBV carriers, but the level of HBV DNA decreases after stopping the drug. Elevated, long-term drug-resistant viruses are prone to drug-resistant mutations. The purpose of the present invention is to develop a new drug target specifically for the replication of hepatitis B virus, and this drug can inhibit the replication of all hepatitis-like viruses

Method used

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  • Usage of caffeine and its analogue in resistance of hepatitis B replication and use thereof in preparation of medicament for treating viral hepatitis
  • Usage of caffeine and its analogue in resistance of hepatitis B replication and use thereof in preparation of medicament for treating viral hepatitis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: The replication of hepatitis B virus depends on the activation of DNA damage pathway

[0030] 1. Materials

[0031] Cell culture plates and other consumables were purchased from Huamei Company. HL7702 cells were purchased from the Cell Preservation Center of Shanghai Institute of Biochemistry, Chinese Academy of Sciences, and hepatitis B virus positive serum was provided by the Laboratory Department of Armed Police General Hospital. The HBV DNA titer of the serum was 5×10 by fluorescent quantitative PCR 7 .

[0032] Caffeine and theophylline were purchased from SIGMA. All antibodies were purchased from cell signaling company. The hepatitis B virus DNA detection kit was purchased from Beijing Dongsheng Biological Co., Ltd.

[0033] 2. Method

[0034] 1. Hepatitis B virus infection of HL7702 cells: 24 hours after the HL7702 cells were transferred to a 6CM plate, the HBV infection test was performed. The test was divided into a negative control group and an...

Embodiment 2

[0039] Example 2: Caffeine and theophylline can inhibit HepG2 2215 cells from secreting hepatitis B surface antigen

[0040] 1. Materials

[0041] Caffeine and theophylline were purchased from SIGMA;

[0042] FBS fetal bovine serum was purchased from Hangzhou Sijiqing. DMEM medium was purchased from GIBCOL company

[0043] HepG2 2-2-15 was preserved by our laboratory. IFN-r was purchased from Sanyuan Gene, and lamivudine was purchased from Jiashitang Pharmacy.

[0044] Hepatitis B surface antigen and core antigen diagnostic kits were purchased from Huamei Bioengineering Company.

[0045] 2. Method

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PUM

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Abstract

The invention discloses an application of caffeine and analogue in the virus hepatitis drug, which is characterized by the following: improving antiviral effect; inhibiting the duplication of variation hepatitis B virus.

Description

technical field [0001] The invention relates to the anti-replication effect of caffeine and its analogs on hepatitis B virus and its application in the preparation of medicines for treating viral hepatitis, belonging to the field of medicine. Background technique [0002] Hepatitis B virus (HBV) itself cannot directly cause liver cell lesions, and the pathogenesis of hepatitis (ie, necrosis and inflammation of liver tissue) is the result of the immune clearance process initiated by the human body after HBV infection. If the immune mechanism of the human body cannot eliminate the persistent existence and active replication of HBV in the body in time, it will inevitably lead to the continuous progression of liver tissue damage. Therefore, in order to prevent the progressive damage of the liver, effective drugs must be taken to eliminate or inhibit the replication of HBV. That is to say, antiviral therapy is a key measure to control chronic hepatitis B. [0003] At present, th...

Claims

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Application Information

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IPC IPC(8): A61K31/522A61K9/08A61K9/19A61K9/20A61P1/16A61P31/20
Inventor 钟辉马清钧张艳红赵帆侯宁波何湘
Owner INST OF BIOENG ACAD OF MILITARY MEDICAL SCI OF THE CHINESE
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